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作者(中文):許珮綺
作者(外文):Hsu, Pei-Chi
論文名稱(中文):拮抗木瓜炭疽病之酵母菌篩選與抗菌物質特性分析
論文名稱(外文):Screening and characterization of antifungal components produced by yeasts against Colletotrichum boninense.
指導教授(中文):李清福
指導教授(外文):Lee, Ching-Fu
學位類別:碩士
校院名稱:國立新竹教育大學
系所名稱:應用科學系碩士班
學號:10225002
出版年(民國):105
畢業學年度:104
語文別:中文
論文頁數:75
中文關鍵詞:木瓜炭疽病拮抗酵母菌
外文關鍵詞:papayaanthracnoseantagonistic yeasts
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目錄.I
圖表目錄.IV
中文摘要.V
英文摘要(Abstract).VI
前言.1
前人研究.3
材料與方法.7
一、實驗用藥品.7
二、培養基及相關藥品配置方法.7
三、器材.9
四、儀器.9
五、酵母菌分離與鑑定.10
(一)樣品收集.10
(二)酵母菌分離.10
(三)酵母菌菌種分子鑑定.10
(四)生理生化試驗.15
(五)形態觀察.16
六、酵母菌保存.18
七、木瓜炭疽病病原菌活化、覆核與病原性試驗.18
(一)木瓜炭疽病病原菌收集及活化.18
(二)木瓜炭疽病病原菌大量與保存.18
(三)病原菌分子鑑定.18
(四)病原菌形態特徵分析與觀察.19
(五)木瓜炭疽病病原性確定.19
八、拮抗酵母菌之篩選.19
九、拮抗酵母菌抑菌能力試驗.20
(一)菌體抑制試驗.20
(二)對峙試驗.20
(三)培養液擴散試驗.20
(四)黴菌生長試驗.21
十、拮抗酵母菌之最適萃取拮抗物質條件.21
(一)萃取方式.21
(二)pH.21
(三)Triton.21
十一、抗菌物質最適生產培養條件.21
(一)基本培養方法.22
(二)化學分析方法.22
(三)不同碳源探討.22
(四)不同探源濃度探討.22
(五)不同碳氮源比例探討.22
(六)不同時間生產拮抗物質探討.23
十二、拮抗物質活性影響因子測試.23
(一)溫度穩定性.23
(二)溫度反應性.23
(三)pH反應性.23
(四)pH穩定性.23
十三、拮抗物質純化.23
十四、拮抗廣效性測試.24
(一)細菌組試驗.24
(二)絲狀真菌試驗.24
(三)酵母菌試驗.24
結果.26
一、植物病原菌覆核、鑑定及致病性確認.26
(一)病原菌覆核及鑑定.26
(二)病原菌致病性確認.26
二、拮抗酵母菌篩選與鑑定.26
(一)酵母菌篩選.26
(二)拮抗酵母菌菌種鑑定.30
三、拮抗酵母菌抗菌活性分析.40
(一)對峙試驗.40
(二)菌體抑菌試驗.45
(三)黴菌生長抑制能力試驗.45
(四)培養液抑菌能力試驗.45
四、拮抗酵母菌最適萃取抗菌物質條件.48
(一)不同pH值緩衝液下之萃取試驗.48
(二)介面活性劑濃度對抗菌物質萃取影響.48
五、最適生產條件之培養基.52
(一)不同碳原利用性.52
(二)不同碳原濃度.52
(三)不同碳氮原比例.55
(四)培養時間.55
六、抗菌物質之特性析.55
(一)溫度穩定性.55
(二)溫度反應性.59
(三) pH穩定性.59
(四) pH反應性.59
七、抗菌物質分離及純化.59
八、純化物質拮抗廣效性之抑菌分析.62
討論.66
結論.69
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封面目錄摘要
前言前人研究
材料與方法
結果
討論
結論
參考文獻
 
 
 
 
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