摘要
目的：探討補充樟芝菌絲體對衰竭運動後大鼠血液生化指標之影響。方法：40隻雄性大鼠隨機分成四組，分別為控制組（C）、衰竭運動組（E）、樟芝組（A）和樟芝衰竭運動組（AE）。A及AE組依照大鼠每公斤體重每天投予0.3克樟芝，為期四週；E及AE組以漸增式衰竭運動模式跑至衰竭。以獨立樣本t檢定分析運動時間，再以二因子變異數分析血液生化指標包括：血糖（Glu）、乳酸（Lac）、乳酸脫氫酶（LDH）、肌酸激酶（CK）、丙二醛（MDA）、超氧離子歧化酶（SOD）、麩胱甘肽過氧化酶（GSH-Px）、麥胺草醋酸轉胺酶（AST）、麥胺丙酮酸轉胺酶（ALT）、三酸甘油酯（TG）、總膽固醇（TC）、高密度脂蛋白膽固醇（HDL-C）及低密度脂蛋白膽固醇（LDL-C）。結果：AE組運動時間顯著高於E組，E組的Lac、LDH、CK、MDA及ALT數據顯著高於C組及AE組，AST活性顯著高於C組，Glu、SOD、GSH-Px、TG及TC數值顯著低於C組，SOD活性顯著低於AE組。A組的Glu、SOD活性顯著高於C組，GSH-Px活性顯著高於AE組，MDA及CK值顯著低於AE組。結論：本研究推論補充樟芝菌絲體有助於減緩大鼠因衰竭運動後造成疲勞之現象，降低肌肉損傷，減少氧化壓力及肝損傷之情形，且維持血糖穩定，使運動時間延長。

Abstract
Purpose: The purpose of this study examined the effects of Antrodia camphorata supplementation on blood biochemical parameters after exhaustive exercise in rats. Methods: Forty male Sprague-Dawley rats were randomly divided into four groups: control (C, n = 10), exhaustive exercise (E, n = 10), A. camphorata supplemented (A, n = 10) and A. camphorata plus exhaustive exercise (AE, n = 10). A. camphorata was supplemented at the dose for four weeks. Exhaustive exercise was performed on a rodent treadmill with the progressive protocol. Two way ANOVA and t-test were performed to examine the effects of exhaustive exercise and A. camphorata supplementation on exercise duration, glucose (Glu), Lactate (Lac), lactate dehydrogenase (LDH), creatine kinase (CK), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), and low density lipoprotein-cholesterol (LDL-C) levels. Results: The plasma Lac, LDH, CK, MDA, and ALT levels in E group were significantly higher than C and AE group, AST activities were significantly higher than C group, while the level of Glu, SOD, GSH-Px, TG and TC were significantly lower than C group, and the plasma SOD activities were significantly lower than AE group. The level of Glu and SOD in A group was significantly higher than C group, while the level of GSH-Px levels were significantly higher than AE group, and the plasma MDA and CK levels were significantly lower than AE group. Conclusions: It was inferred from the experiment that A. camphorata mycelium supplementation could be beneficial for alleviating the fatigue after exhaustive exercise in rat, and reduce muscle damage, as well as decrease the oxidative stress and harm on liver function. It also maintains the blood glucose level, which results in prolonging the duration of endurance exercise.

目　錄
口試委員與系主任簽字之論文通過簽名表 I
論文授權書 II
中文摘要 III
英文摘要 IV
謝誌 V
目次 VI
表次 VIII
圖次 IX
第壹章　緒論 1
第一節　問題背景 1
第二節　研究目的 3
第三節　研究假設 4
第四節　名詞操作性定義 4
第五節　研究範圍與限制 6
第六節　研究的重要性 6
第貳章　文獻探討 7
第一節　運動與疲勞 7
第二節　運動與肌肉損傷 8
第三節　運動與氧化壓力 9
第四節　運動與肝損傷指標 11
第五節　運動與血脂肪 12
第六節　樟芝與血液生化指標 12
第七節　本章小節 13
第參章　研究方法 15
第一節　實驗對象 15
第二節　材料與儀器 15
第三節　實驗設計 17
第四節　實驗流程 19
第五節　生化分析 20
第六節　資料處理 20
第肆章　結果 21
第一節　疲勞指標 21
第二節　肌肉損傷指標 23
第三節　氧化壓力指標 25
第四節　肝損傷指標 28
第五節　血脂肪指標 30
第伍章　討論 32
第一節　樟芝補充與疲勞 32
第二節　樟芝補充與肌肉損傷 33
第三節　樟芝補充與氧化壓力 33
第四節　樟芝補充與肝損傷指標 34
第五節　樟芝補充與血脂肪 35
第陸章　結論與建議 37
參考文獻 38


表　次
表4-1-1　各組大鼠運動時間、血糖及乳酸之數據....................................................21
表4-2-1　各組大鼠乳酸脫氫酶及肌酸激酶的活性..................................................23
表4-3-1　各組大鼠丙二醛、超氧離子歧化酶及麩胱甘肽過氧化酶之數據............25
表4-4-1　各組大鼠麥胺草醋酸轉胺酶及麥胺丙酮酸轉胺酶的活性......................28
表4-5-1　各組大鼠三酸甘油酯、總膽固醇、高密度脂蛋白膽固醇與
低密度脂蛋白膽固醇濃度 30
 
圖　次
圖2-3-1　自由基與抗氧化清除作用 11
圖3-2-1　老鼠電動跑步機 16
圖3-3-1　餵食老鼠之情形 17
圖3-3-2　大鼠漸增式衰竭運動處方 18
圖3-4-1　實驗流程圖 19
圖4-1-1　衰竭運動與樟芝補充對血糖濃度的影響 22
圖4-1-2　衰竭運動與樟芝補充對乳酸濃度的影響 22
圖4-2-1　衰竭運動與樟芝補充對乳酸脫氫酶活性的影響 23
圖4-2-2　衰竭運動與樟芝補充對肌酸激酶活性的影響 24
圖4-3-1　衰竭運動與樟芝補充對丙二醛濃度的影響 26
圖4-3-2　衰竭運動與樟芝補充對超氧離子歧化酶活性的影響 26
圖4-3-3　衰竭運動與樟芝補充對麩胱甘肽過氧化酶活性的影響 27
圖4-4-1　衰竭運動與樟芝補充對麥胺草醋酸轉胺酶活性的影響 29
圖4-4-2　衰竭運動與樟芝補充對麥胺丙酮酸轉胺酶活性的影響 29
圖4-5-1　衰竭運動與樟芝補充對三酸甘油酯濃度的影響 31
圖4-5-2　衰竭運動與樟芝補充對總膽固醇濃度的影響 31

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