本研究為液滴微流控製備分子拓印微珠，達到微小化及其應用於食品藥物殘留檢側，研究內容包含微流道晶片設計、晶片製程與封裝、溶液性質探討、微液滴形成參數探討如流道設計、兩相流率比及錶面活性劑濃度、微粒固化、藥物吸附性測試如專一性與選擇性以及微粒管柱。
微流道設計，透過繪圖軟體AutoCAD進行光罩設計，並使用微影製程製作微流道晶片，藉由真空氧電漿進行晶片黏合，並以注射式幫浦與攝影機進行微流體驅動及微液滴觀測。本研究針對微液滴形成進行相關參數探討，由結果得知在頸為50 µm時，可以產生微小且均勻為液滴；另外，在固定乳化相微流量為6 µL/hr而連續相微流量為30~600 µL/hr下，隨著連續相加快，產生液滴尺寸也越小，最小可到達15μm。在微液滴固化實驗方面，使用UV曝光機照射紫外光(λ=365 nm)，當固化能量達到60 W/cm2時，微液滴可以固化形成微粒，以上為本論文製備分子拓印微粒程序。本論文開發微小化分子拓印微粒應用於食品藥物殘留檢側上，由實驗結果可知，針對Ractopamine與Doxycycline兩種藥物拓印微粒之吸附測試，其專一性吸附經過調整最高可以到達580%與415%與選擇係數分別為17.07(DC)、3.68(PEN)與6.65(RAC)、2.06(PEN)，吸附時間為60秒。微粒微小化對於藥物吸附量測試，當粒徑單位重量之總表面積提升8.9倍時，吸附分子量也增加1.86倍。整合微粒於微流體晶片形成微粒管柱，探討不同流道貼紙厚度與分散液體積，最佳測試結果流道貼紙厚度與分散液體積分別為120 μm與0.5 mL。本論文所開發之微液滴晶片能夠提供分子拓印微液滴產生與合成最小粒徑的微粒，且未來可以應用於合成奈米材料如奈米碳管

In this thesis, molecularly-imprinted polymer beads fabricated by emulsion droplet methods to realize the tiny beads and their applications to Detection of Drug Residue in Foods. The study contain simulation, the design of microchannel、the chips are fabricated and characterized. Then, discussion on solution property (dielectric constant and chemical structure). The behavior of droplet formation, such as the omicrochannel width, two-phase flow ratio, surfactant consentration, particulate polymerization, drug adsorption testing such as specificity and selectivity, and particulate package, have been well investigated.
The microchannel designs are draw by using AutoCAD .In the experiment, the chips are fabricated by using photolithography process, and then oxygen plasma for bonding. The experimental results show that tiny and homogeneous droplets can be generated, as the microchannel width equal to 50 µm ; droplets decreases as the flow rate of continuous phase increases under the flow conditions of 6 µL/hr for emulsion phase and 30~600 µL/hr for carrier phase, The smallest particle size can reach 15μm. In the droplets polymerization experiment. As the curing energy reaches 60 W / cm2, the micro-droplet can be polymerizated to form microparticles by using ultraviolet light (λ = 365 nm).According to experiental result, the specificity of the developed Ractopamine-imprinted beads is up to 580% and selectivity coefficient equal to 17.07(DC)、3.68(PEN); The specificity of the developed Doxycycline-imprinted beads is up to 415% and selectivity coefficient equal to 6.65(RAC)、2.06(PEN), the adsorption time is 60 seconds. When the total surface area per unit weight of the particle is increased by 8.9 times, the molecular weight of the adsorbed molecule increases by 1.86 times. The microbeads form a column on-chip, when the thickness of the stickers and the volume of the dispersion liquid were 120 μm and 0.5 mL, respectively.
The developed droplet chips in this research have the potenials of hight-througput droplets and synthesis the smallest MIP beads.In future, the droplet chips can be fuether synthesis of nanomaterials, such as carbon nanotube.

目錄
中文摘要 iv
ABSTRACT vi
誌謝 viii
目錄 ix
圖目錄 xii
表目錄 xvii
第一章 緒論 1
1.1 研究背景 1
1.2 分子拓印高分子 11
1.2.1. 分子拓印高分子原理 11
1.2.2. 分子拓印高分子合成 15
1.2.3. 分子拓印高分子應用 17
1.3 乳化微液滴 19
1.3.1. 微液滴發展 19
1.3.2. 微液滴操控方式 23
1.3.3. 微液滴應用於高分子微粒形成 27
1.4 研究動機 31
1.5 研究目的與方法 32
1.6 論文計劃書架構 33
第二章 微液滴形成性質 34
2.1. 微液滴形成物理性質影響 34
2.1.1. 無因次參數探討 34
2.1.2. 流道寬度與厚度影響 36
2.1.3. 驅動流體壓力 37
2.2. 微液滴形成化學性質影響 39
2.2.1. 表面活性劑原理與分類 39
2.2.2. 微流道表面性質 44
2.3. 分子拓印溶液物理性質與化學性質探討 48
2.3.1. 分子拓印溶液物理參數探討 48
2.3.2. 流道表面官能基與分子拓印溶液鍵結探討 49
第三章 分子拓印高分子微液滴形成實驗 51
3.1. 微流道設計與製作 51
3.1.1. 微流道設計與負光阻製程 51
3.1.2. 微流道封裝 55
3.2. 乳化微液滴平台架設 57
3.3. 乳化微液滴參數探討 58
3.3.1. 水-油之微液滴微小化與均勻化探討 58
3.3.2. 流道表面修飾於乳化分子拓印溶液沾粘性與液滴穩定性探討 64
3.3.3. 微流道頸口尺寸與分子拓印微液滴均勻性測試 68
3.3.4. 兩相流率比與分子拓印微液滴尺寸影響 71
3.3.5. 表面活性劑比例於液滴穩定性影響 74
3.4. 結論 76
第四章 分子拓印高分子微粒固化實驗 78
4.1. 懸浮聚合微粒 78
4.1.1. 熱聚合固化參數探討 79
4.1.2. 藥物分子拓印微粒形態與粒徑分佈 82
4.2. 微液滴聚合微粒 84
4.2.1. 光固化能量探討 86
4.2.2. 藥物分子拓印微粒形貌與粒徑分佈 88
4.3. 結論 90
第五章 分子拓印微粒於食品殘留藥物檢測之應用 91
5.1. 分子拓印微粒於食品殘留藥物檢測之應用 91
5.2. 分子拓印高分子微粒藥物檢測實驗 93
5.2.1. 待測肉汁前處理程序 93
5.2.2. 藥物拓印微粒清洗次數 93
5.2.3. 藥物拓印微粒專一性吸附測試 96
5.2.4. 藥物拓印微粒吸附時間影響 101
5.2.5. 藥物拓印微粒選擇性測試 103
5.3. 全整合於晶片上之分子拓印管柱 107
5.3.1. 整合分子拓印管柱之檢測架構 107
5.3.2. 藥物拓印微粒管柱填充 109
5.4. 超聲波檢測訊號 113
5.5. 結論 115
第六章 總結與研究成果 116
6.1. 總結 116
6.2. 研究成果 117
6.3. 學術貢獻 119
6.4. 未來研究建議 126
參考文獻 130
附錄 140
A 建構實現於陣列式多組藥物拓印高分子微粒之微幫浦驅動系統 140
A.1 微型幫浦之壓力量測 140
A.2 定壓力驅動-乳化油水微液滴測試 144
B. 藥物性質列表 149
C. 量測儀器資訊 145
發表著作 151
作者簡介 152

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