人體中不正常的多巴胺含量與某些疾病息息相關，傳統多巴胺的感測常面臨干擾物和生物膜汙染等問題而失準。本研究以偏壓輔助化學氣相沉積法製備導電性超奈米微晶鑽石(nitrogen-incorporated ultrananocrystalline diamond, NUNCD)薄膜，其方法為在氮氣電漿下，以電漿功率1400 W、基板偏壓-300 V、腔體壓力50 torr的條件成長一小時，所得的NUNCD之電阻率約為63.15 μΩ·cm。接著我們使用此NUNCD做為工作電極，以電化學的方式對多巴胺進行感測，偵測極限可以達到0.32 μM。即使在具有氧化電位相近的干擾物（抗壞血酸及尿酸）環境下，偵測極限依然不受影響。在胎牛血清中，NUNCD對多巴胺的偵測亦能得到良好的線性區間，偵測極限為0.39 μM，僅略差於在磷酸鹽緩衝溶液中的量測值。最後建立多巴胺峰值電流對濃度的檢量線，並實際將微分脈衝伏安法量測所得的電流值，帶入檢量線中，換算待測物所含的多巴胺濃度，將其與實際加入的多巴胺濃度做比較，得到約90-120%的回收率(recovery)，顯示本研究所製備的NUNCD電極對多巴胺的感測有相當的可行性。

Abnormal dopamine level in human body is related to some diseases. However, traditional dopamine sensing methods were confronted with problems such as interference and biofilm-fouling. This study adopted electrochemical method to detect dopamine, and the electrode we used was conductive nitrogen-incorporated ultrananocrystalline diamond (NUNCD) films which were prepared through the biased enhanced growth method. The synthesis conditions for NUNCD were 1400 W plasma power, -300 V applied bias at the substrate, 50 torr chamber pressure, and deposition for 1 hour. The best resistivity of the film was about 63.15 μΩ·cm. For the dopamine detection in the phosphate buffer solution, the detection limit was about 0.32 μM. In addition, detection of dopamine in the presence of ascorbic acid and uric acid were performed and it is found that the detection limit was not affected by the interference of biomolecules and the electrode showed good selectivity. In fetal bovine serum, the electrode also performed excellently with a detection limit of about 0.39 μM. We also built up calibration curves and equations to calculate the content of the dopamine in a blind sample. By substituted the current, obtained from the result of differential pulse voltammetry, into the equation and calculated the dopamine level, we found the dopamine concentration calculated from the equation has the same level with the value we added. Recovery of the electrode is about 90-120%, indicating that the NUNCD film has great potential to be used as an electrode for dopamine sensing.

摘要 I
Abstract II
致謝 III
目錄 IV
表目錄 VIII
圖目錄 IX
第一章　緒論 1
第二章　文獻回顧 3
2.1 多巴胺之感測 3
2.1.1 化學感測器 3
2.1.2 感測器之要素 3
2.1.3 傳統多巴胺感測方法 4
2.1.4 電化學量測 5
2.1.5 生物分子的電化學性質 6
2.1.6 多巴胺量測的困難 9
2.2 鑽石薄膜之簡介 10
2.2.1 鑽石的特性及應用 11
2.2.2 人工鑽石之發展進程 12
2.2.3 基板的選擇 12
2.2.4 基板前處理 13
2.2.5 鑽石薄膜的分類 14
2.2.6 NUCND 15
2.2.7 鑽石的分析方法 17
第三章　實驗分析及方法 28
3.1 鑽石薄膜的成長 28
3.1.1 試片前處理 28
3.1.2 沉積鑽石薄膜 28
3.2 電化學量測 29
3.2.1 伏安法的量測步驟及參數 30
3.2.2 待測樣品的條件 30
3.2.3 回收率的取得 31
3.3 實驗儀器及分析儀器 32
3.3.1 微波電漿輔助化學氣相沉積系統 32
3.3.2 循環伏安法 32
3.3.3 微分脈衝伏安法 33
3.3.4 場發射掃描式電子顯微鏡 34
3.3.5 拉曼光譜分析 34
3.3.6 X射線光電子能譜 35
3.3.7 光放射光譜 35
3.3.8 霍爾量測系統 36
第四章　結果與討論 43
4.1 鑽石薄膜的成長 43
4.1.1 電漿所含成分 43
4.1.2 表面形貌分析 44
4.1.3 拉曼光譜分析 44
4.1.4 XPS 分析 46
4.2 感測生物分子 47
4.2.1 循環伏安法偵測分子的電化學性質 47
4.2.2 循環伏安法和微分脈衝伏安法用於感測生物分子 48
4.2.3 利用微分脈衝伏安法感測多巴胺 49
4.2.4 選擇性測試 50
4.2.5 在胎牛血清中感測多巴胺 52
4.2.6 利用檢量線實際感測多巴胺濃度 53
第五章　結論 69
參考文獻 71

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