福斯特共振能量轉移(Förster resonance energy transfer, FRET)效應被廣泛地應用於生物體醫學感測方面的研究，而在此我們希望能夠運用去氧核醣核酸(deoxyribonucleic acid, DNA)複合材料與光化學還原金奈米粒子的特性，進階擴展FRET效應於光電領域方面的發展。
此篇研究中，我們首先介紹了因DNA特殊雙股螺旋結構的特性，用於染料FRET系統中當作染料的主體可減少染料分子的自消光現象，進而提升FRET系統中螢光染料分子間的能量轉移效率，並以常見的有機高分子聚合物－聚甲基丙烯酸甲酯(PMMA)作為對照組與其比較，發現對於雙染料系統而言，以DNA複合物作為主體的FRET能量轉移效率較以PMMA作為主體的FRET能量轉移效率高。另外，我們使用光化學還原之金奈米粒子的局部表面電漿共振來改變FRET系統的特性，首先利用金奈米粒子的吸收光譜圖探討照光還原的情況，再以TEM電子影像圖討論金奈米粒子藉由不同照光還原時間的生成型態，最後摻雜不同還原時間的金奈米粒子溶液於染料FRET系統中，藉此改變染料FRET系統螢光薄膜的光學特性。我們以螢光光譜圖分析染料FRET系統的螢光特性與CIE色度座標圖來探討FRET系統所顯現的光色變化，發現隨著摻雜的體積比例增加，系統的螢光與光色會有一明顯的變化趨勢，未來可運用於紫外線感測與光電元件上。

Förster resonance energy transfer (FRET) has been widely applied in biomedical sensing studies. The purpose of our study is to explore potential FRET-mediated applications in optoelectronics by combining the fabrication platform of deoxyribonucleic acid (DNA) composite and photoinduced gold nanoparticles (NPs).
In this study, we first examine the FRET efficiency in dye-doped DNA system and show that the efficiency could be enhanced by virtue of the structural features of DNA compared to the PMMA counterparts. It is suggested that the structure of DNA can spatially organize the chromophores and reduce the phenomenon of self-quenching due to the aggregation of chromophores. Furthermore, we examine the influence of localized plasmon resonance in the FRET systems by addition of gold NPs synthesized by photoreduction method. We analyze the optical properties of gold NPs with different photoreduction time by absorption spectra and the shape of gold NPs with different photoreduction time by transmission electron microscopy (TEM). Finally, we investigate the FRET systems with different doping levels of gold NPs by photoluminescence (PL) spectra and color analysis in CIE color space. We present that color of the thin film can be changed with different doping levels of gold NPs by FRET. Such effect can be used in the applications of UV sensors and various photonic devices.

摘要 I
Abstract II
致謝 III
目錄 IV
圖目錄 VI
第1章 文獻回顧與介紹 1
1.1 前言 1
1.2 生物性高分子DNA介紹及應用 1
1.2.1 DNA介紹 1
1.2.2 DNA材料發展與應用 3
1.3 Förster resonance energy transfer (FRET) 6
1.3.1 FRET基本原理 6
1.3.2 FRET效應應用 8
1.4 金屬奈米粒子介紹 12
1.4.1 金屬奈米粒子局部表面電漿共振原理及其應用 12
1.4.2 金屬奈米粒子製備 15
1.5 研究動機 16
第2章 實驗製備與量測 17
2.1. 藥品介紹 17
2.2. DNA複合材料製備 17
2.2.1 DNA-CTMA合成 17
2.2.2 DNA-染料複合材料製備 18
2.2.3 光合成金奈米粒子 19
2.3. 元件製備 20
2.4. FRET效率計算 21
2.5. 儀器介紹 22
3.1 染料螢光薄膜FRET系統 26
3.1.1. DNA與PMMA-染料螢光薄膜 26
3.1.2. 不同主體之FRET能量轉移效率比較 31
3.2 金奈米粒子於染料螢光薄膜FRET系統 32
3.2.1 照光時間對金奈米粒子生成的影響 33
3.2.2 金晶種摻雜濃度的影響 35
3.2.3 金奈米粒子摻雜濃度的影響 41
3.2.2 CIE於FRET系統之分析 47
第3章 總結與未來展望 51
參考文獻 52

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