我們介紹了一種低花費且可表達細菌對於抗生素的進化途徑的培養裝置，這個裝置可連續的觀察細菌的成長過程，為了驗證這個系統，我們逐步的測量Escherichia coli MG 1655對於trimethoprim的抗藥性，並置入多重微流體裝置中並測量大腸桿菌的型態變化和抗藥性，該方法可以延伸到實驗室的抗生素耐藥性研究，並擴展到其他的適應性進化和細菌培養實驗。

We describe a low cost, configurable bacterial culture device for characterizing the evolutionary pathway of antibiotic resistance. This device continuously monitors the evolving bacterial growth. To validate the platform, we measure the stepwise acquisition of trimethoprim resistance in Escherichia coli MG 1655, and integrate the platform into a multiplexed microfluidic platform for investigating cell morphology and antibiotic susceptibility. The approach can be up-scaled to laboratory studies of antibiotic drug resistance, and is extendible to other adaptive evolution and bacterial culture experiments.

誌謝 I
中文摘要 II
Abstract III
目錄 IV
圖目錄 (List of Figure) VII
表目錄 VIII
一、緒論 1
1-1 研究動機 1
1-2 文獻回顧 3
1-2-1 連續性量測 3
1-2-2 傳統抗藥性量測 3
1-2-3 微流體抗藥性測試 4
1-2-4 抗藥性測試 6
1-2-5 Morbidostat 7
二、研究材料 8
2-1 光罩 8
2-2 模仁 9
2-3 閥門 10
2-4 微流體晶片 11
三、研究方法 12
3-1 微流體晶片製作 12
3-1-1 微流體晶片設計 12
3-1-2 模仁製作 14
3-1-3 晶片製作 15
3-2 系統架設 18
3-2-1 硬體系統 18
3-2-2 Labview控制系統 19
3-2-3 溫度控制系統及光學顯微鏡 20
四、實驗結果 21
4-1 大腸桿菌樣品準備 21
4-2 實驗前晶片表面處理及系統參數 21
4-3 實驗結果 23
4-3-1 實驗(一) 24
4-3-2 Cell Area 27
4-3-3 型態變化 30
4-3-4 實驗(二) 31
4-3-5 Cell Area 34
4-3-6 型態變化 37
五、結論 38
附錄 39
中英對照表 44
參考文獻 45

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