人類的大腦是非常精巧和複雜的器官，科學家們為了瞭解大腦的運作，會以簡單的果蠅大腦來進行研究，透過觀察果蠅的行為，加上生物技術之輔助來了解大腦的運作。但現今果蠅的行為實驗平台均為一維或二維的自由度，會造成侷限果蠅的行為，影響分析結果，並且大量的實驗也會耗費大量的人力，因此提出生物球果蠅實驗平台，將實驗所需的果蠅懲罰器與果蠅的學習標的，透過微機電技術和製程整合，製作成透明可撓之多功能薄膜，此薄膜再透過組裝貼附於壓克力透明球內，形成三維的果蠅測試環境，可使果蠅表現更完整的行為，而透明的結構可整合外部影像擷取系統自動化紀錄果蠅之行為，降低人力的消耗。
實驗平台之多功能薄膜為多層膜之結構，其整合電擊和熱刺激式做為果蠅之懲罰，藍光和琥珀光之發光二極體做為果蠅之學習標的，而多層膜之電性走線透過垂直導線，形成具有三維電性連結架構，來完成複雜的走線與電性牽引。未來還可以透過增加薄膜之層數來整合更多刺激與感測元件，使實驗平台之功能更加廣泛、完整。

Drosophila is a well-known vehicle for researchers to investigate the brain diseases, such as Alzheimer's disease and autism. Currently, due to the sensing technologies, most of the existing experiments only allow flies moving in single-dimension (tube) or two-dimension (arena). However, the flies have the native behavior of moving in 3D space. Therefore, this study establishes a bio-sphere Drosophila test platform to realize a natural like testing environment.
This study presents a novel “multi-function bio-sphere Drosophila test platform” implemented by Micro Electro Mechanical System (MEMS) and flexible electronics technology. The bio-sphere is consisted of a transparent rigid acrylic sphere covered with a transparent flexible MEMS bio-film with embedded micro transducers. The transparent bio-film can sense fly’s position, and stimulate flies by giving electric shock and heat. The multi-function bio-sphere test platform provides a natural like testing environment (sphere space) for fly’s behavior observation, and the future potential of automatic training and recording.

中文摘要 i
致謝 iii
目錄 iv
圖目錄 vi
表目錄 xi
第一章 緒論 1
1-1 前言 1
1-2 文獻回顧 4
1-3 研究方法 7
1-3-1 果蠅測試環境空間 7
1-3-2懲罰機制 8
1-3-3學習標的 9
1-3-4 行為及位置感測方式 10
1-4 小結 11
第二章 生物球設計與初步測試 26
2-1 生物球規劃 26
2-1-1生物球結構 26
2-1-2懲罰薄膜貼附圖形設計 27
2-1-3 懲罰薄膜設計 28
2-3 懲罰薄膜測試結果 30
2-4 小結 34
第三章 多功能薄膜設計與製程結果 51
3-1 多功能薄膜設計規劃 51
3-1-1 多功能薄膜材料選擇 52
3-1-2 多功能薄膜懲罰器與學習標的設計 53
3-1-3 焊墊與拉線連接之改善 55
3-2 多功能薄膜製作 55
3-3 多功能薄膜製程結果與討論 57
3-4 多功能薄膜測試結果 59
3-5 多功能薄膜問題與改善方案 60
第四章 結論與未來工作 79
4-1 結論 79
4-2 未來工作 79
第五章 參考文獻 84

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