本研究是利用光化學方法合成近紅外光敏感之金/磁赤鐵礦核/層奈米材料及研究其應用，此種核/層奈米結構有導體/半導體接點，能（a）增強電漿引起電荷分離之效率及（b）使其吸光光譜移到太陽光波長或生物可穿透的紅外線範圍，使它們成為有潛力的太陽能組件或是癌症光動力療法之感光劑。此研究是將光合成奈米材料用於抑制癌症及抗菌的感光劑，並和同樣大小的純奈米金作比較，藉此展現光合成奈米材料能產生更大量自由基殺死癌細胞而非透過熱。結論是本篇證明了激發光合成奈米材料可達到光動力療法殺死癌細胞，而並非只有光熱效應被考慮。且此產生自由基的能力也可用於抗菌。

A photochemical method of preparing gold-maghemite core-shell nanoparticles by using artificial lamps or sunlight is developed in this research. Core-shell metal-semiconductor nanoparticles possess (a) Schottky junctions which can enhance plasmonic induced charge separations and (b) tailored absorbance spectra for absorbing solar light and tissue-penetrable near-infrared (NIR), so they are potentially useful and important for solar energy applications or cancer phototherapy. In our work, the photosynthesized core-shell nanoparticles (PCNPs) was applied for cancer phototherapy and antimicrobial agent. By comparing with similar-sized gold nanoparticles (AuNPs), we highlighted that the photogenerated radicals of PCNPs within cells can cause the cell death more efficiently instead of heat. Thus, it is a demonstration of photodynamic therapy (PDT) achieved by irradiation of PCNPs instead of photothermal therapy (PTT). And the radical generation ability also showed quite antimicrobial efficiency.

Abstract 1
摘要 2
Acknowledgement 3
Table of Contents 4
List of Figures 6
1. Introduction 9
1.1 Photochemical Reactions 9
1.2 Optical Properties of Metallic Nanoparticles 11
1.3 Applications of Optical Properties of Nanoparticles 13
1.3.1 Gold Nanoparticles as Photothermal Thermal (PTT) Sensitizers 13
1.3.2 Nanoparticles and Photodynamic Therapies 14
1.3.3 Solar Cells or Water Splitting Devices Applying Nanoparticles as Sensitizers 15
1.4 Research Motivation 18
2. Material and Methods 20
2.1 Materials 20
2.2 Experiment Procedures 21
2.2.1 Preparation of Colloidal Gold 21
2.2.2 Preparation of Gold Nanoparticles (AuNPs) 21
2.2.3 Preparation of Ferrocenium Salt 21
2.2.4 Photosynthesis of Gold-Maghemite Core-Shell Nanoparticles (PCNPs) 21
2.2.5 Cyclic Voltammetry Measurements and Preparation of AuNP Coated Electrode 22
2.2.6 Characterizations of Nanoparticles 22
2.2.7 Photocatalytic Dye Degradation 23
2.2.8 Photocatalytic Hydroxylation of Terephthalic Acid 23
2.2.9 Electron Paramagnetic Resonance Spin Trapping Experiments 23
2.2.10 Cell Preparetions and Photodynamic Therapy 23
2.2.11 Intracellular ROS Measurements 24
2.2.12 Photocatalytic Antimicrobial Efficiency 24
3. Results and Discussion 25
3.1 Preparation and Characterization of PCNPs 25
3.2 AuNP-O2 Induced Spontaneous Decomposition of Ferrocenium Ion 30
3.3 Electron Transfer from Ferrocene to AuNP-O2 35
3.4 Photocatalytic Dye Degradation and Hydroxyl Radical Generation of PCNPs 41
3.5 In Vitro Phototherapies Sensitized by Nanoparticles 45
3.6 Photosensitized Nanoparticles as Antimicrobial Agents 48
3.7 Photoinduced Charge Separations and Transfer 49
4. Conclusions 51
5. Reference 52
6. Supplementary Information 56

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