表面電漿生物感測技術擁有比其他生物感測方法有更高的靈敏度，如今已經發展出很多不同的表面電漿生物感測方法。本論文主要是利用光柵式表面電漿感測方法偵測生物檢體。此方法最主要是藉由觀察反射頻譜的變化量，而去感測光柵表面物質的變異。我們利用有限時域分析法 (Finite Difference Time Domain)這個方法去模擬後發現改變光柵表面形貌會影響反射頻譜的特性。接著我們將表面電漿晶片整合在微流道上利用微流道能夠大幅減少檢體用量和使液體傳輸自動化的特性使整個偵測流程更加快速方便。再來我們利用整合微流道的表面電漿晶片偵測糖尿病的指標HbA1c 生物檢體。一開始我們先利用螢光標定的方式確認第一層的適體(aptamer)有最好的自組裝，確認好最佳濃度和時間後，我們分別通入0~3 μg/μl 的HbA1c 接著在通入0.025 μg/μl 糖化血紅素抗體(HbA1c antibody)，而我們可以看到HbA1c的訊號偏移量會隨著濃度改變而有不同、而HbA1c antibody 雖然是注入的相同濃度，但會因為HbA1c 的鍵結量不同而有線性變化。

Surface plasmon utilized for biosensing applications has been demonstrated with high sensitivity. There are many types of surface plasmon biosensors today. In this research, we focus on a grating-based surface plasmon biosensor. The grating-based SP biosensor detects the biomolecule through interrogating the shift of the reflection spectrum. According to the simulation result by Finite Difference Time Domain (FTDT) method, we found that the bandwidth is generally related to the profile of the grating. In addition, we integrate microfluidic systems into the SPR chip. Microfluidic systems have the advantage of reducing the usage of specimen and facilitating the delivery of liquid through the entire detection process quickly and easily. After optimizing the concentration and process time of applying HbA1c-specific aptamers, we passed through 0~3 μg/μl of HbA1c, followed by injecting 0.025 μg/μl of HbA1c antibody. We observed the SPR wavelength shifts along with the concentrations of HbA1c, showing a good linear correlation. We also demonstrated that the detection system can be miniaturized for a portable apparatus.

摘要 I
Abstract II
致謝 III
目錄 IV
第一章 緒論 1
1.1適體 1
1.2微流體生醫晶片 3
1.3表面電漿 4
1.4 研究動機 6
1.5 論文架構 7
第二章 理論背景 8
2.1 表面電漿 8
2.2表面電漿晶體(Plasmonic Crystal) 13
2.3 表面電漿做生物量測之原理 14
2.4 微流道工作原理 16
第三章 元件模擬與設計 18
3.1時域有限差分法 18
3.2 光柵設計與模擬 27
第四章 元件製作 33
4.1光柵製作流程 33
4.2 微流道製作 41
第五章 元件量測與分析 46
5.1 光學架構和量測方式 46
5.2 元件特性量測 47
5.3 微流道特性量測 48
5.4 Aptamer優化鍵結 49
5.5 SPR晶片HbA1c量測 54
第六章 結果與討論 57
6.1結果與討論 57
參考文獻 60

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