最近幾年，有一種新的癌症治療方式被提出，也就是癌症治療電場(Tumor Treating Field, TTF)，其在癌細胞分裂時，在週圍加上一頻率的交流電場，抑制癌細胞的分裂，以達到治療的效果。癌症治療電場對應不同的癌細胞，有各自對應的電場頻率，產生的效果才會最大，由於有許多的變因，包括電場施加方向、電場強度與電場施加時間等等，為了方便研究各個細胞對應的電場頻率，故作者設計一晶片，盡量降低其他因素的影響，針對其各自對應的交流電場頻率做研究。
作者設計的癌症治療電場頻率研究晶片，主要有兩個功能，分別是細胞分選與限制細胞分裂方向後施加電場。細胞分選的方式，是利用介電泳力，挑選想要的癌細胞，將癌細胞因開啟的電極不同，分選進入不同的微流道。第二個功能的部分，是利用微結構造成的流阻差別，將許多的單一細胞卡在微流道結構上，在細胞貼附生長後，藉由物理方式的限制，影響細胞的分裂使其只能往特定方向分裂，並在此方向上加上特定頻率的交流電場，來達到癌症治療電場的抑制分裂效果。綜合以上的設計，使得能在單一晶片上，達到對同一細胞，施以不同頻率的電場，或是對不同細胞施以相同頻率的電場的情況，以利於對於不同癌症細胞，各自特定的癌症治療電場頻率的研究。

Tumor Treating Fields (TTF), a technology using alternate current electric fields to treat tumor, was first reported by Y. Palti in 2004. Despite this new technology shows great promising and is being used in the late stage of clinical trials, some of its important mechanisms have not been clearly investigated such as the frequency of the Tumor Treating Field effect.
In this research studies, the microsystem chip was designed for two functions including cell sorting and the optimal Tumor Treating Field (TTF) frequency to each cancer cell. The first function on our microsystem chip is the cell sorting by using negative dielectrophoresis to sort the cells. The individual cell was then guided into the PDMS microchannel. The second part is the special shape of PDMS microstructure. This microstructure has two features, the cell trapping and the direction restriction of the cell division. After cell culture, we built the electric field with different frequency to observe the TTF effect on the cancer cells. This microfluidic platform could be applied to the cell biology and bio-mechanical studies on cells under TTF treatments.

目 錄
Abstract II
中文摘要 III
致 謝 IV
目 錄 VI
圖目錄 IX
一、緒論 1
1.1 前言 1
1.2 現今主要癌症治療方法 2
1.2.1手術切除 2
1.2.2化學療法 2
1.2.3放射線治療 3
1.2.4標靶治療 4
1.3 研究背景與動機 5
1.4 文獻回顧 6
1.4.1 癌症治療電場 6
1.4.1.1 細胞有絲分裂( mitosis ) 7
1.4.1.2 癌症治療電場研究 10
1.4.1.3 作用原理 13
1.4.2 細胞分選 14
1.4.2.1 微流道結構 15
1.4.2.2 陣列基板 17
1.4.3 微流道結構抓取細胞或粒子 21
1.4.4介電泳 25
二、晶片設計與原理 27
2.1 設計基礎與理論 28
2.1.1介電泳力 28
2.1.1.1介電材質其極化機制 28
2.1.1.2介電泳力作用機制 30
2.1.2微流體流道分析 35
2.2設計概念 37
2.2.1細胞分選 38
2.2.2細胞抓取與培養 48
三、實驗材料與晶片製程 54
3.1 晶片製程 54
3.1.1 ITO導電玻璃 54
3.1.2 圖樣電極製程 55
3.1.3 流道結構模型建成 57
3.2 晶片組合 59
四、實驗過程與結果 61
4.1 實驗準備與架設 61
4.2實驗流程 62
4.3實驗結果 63
4.3.1細胞分選 63
4.3.2 抓取培養細胞 65
五、結論 67
參考文獻 68

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