光熱療法(Photothermal therapy, PTT)是近年來在臨床醫學研究領域備受注目的新興的治療方式。不僅能取代化學療法與放射線療法殺死癌細胞，更能夠有效的抑制細菌生長。光熱療法以近紅外線區域的波長照射對光熱感材料使其升溫的利用高溫進行治療。由於雷射的高穿透性及準直性，配合光熱材料，可將治療區域控制在患部，進行有效率的治療。因此廣受醫學界重視。本實驗目標為利用利用以微機電製程技術製作聚吡咯微針貼片，建立一個新型的光熱治療裝置。
本實驗將配合近年來微機電(Micro Electro Mechanical Systems，MEMS)製程技術的進步與普及，發展的新的醫療器材技術—微針(microneedle)。配合過去研究多著重在其導電的聚合物聚吡咯，利用其優異的光熱轉換能力、光穩定性，與配合其良好的生物相容性，製作一種新型光熱療法載體。
在體外實驗中，我們透過調整功率調控聚吡咯微針溫度。以0.5 W/cm2，波長808奈米的近紅外光雷射配合聚吡咯微針進行光熱治療。在動物實驗中，我們證明此裝置有良好的抑制細菌效果，顯示以聚吡咯微針貼片搭配近紅外線照射的光熱治療，是一種相當具有潛力的微生物感染治療方法。
本實驗提供了一種新型的光熱治療模式，此治療方法製作方法簡單，操作簡便且安全。利用聚吡咯光熱效應產生高溫治療細菌感染，既有效且沒有藥物累積性，使用微針進行不會碰觸到痛覺神經達到疼痛，對於患者更無負擔。另外這種產生熱的微針貼片，也提供了微針治療除了藥物傳輸外一種新穎的應用方法。

Photothermal therapy has been highly noticed as a new treatment method in research fields recently. This method is by using photothermal sensitive material with near infrared (IR) light. Those materials have great absorbance of near IR, and can transform the light energy into thermal energy. Provide a new way to treat cancer cells or bacteria infection. Due to the high penetration of laser and its collimation, combining with photothermal sensitive material can produce heat for the efficient treatment. Our goal is to combine the MEMS (Micro Electro Mechanical Systems, MEMS) technology to produce polypyrrole microneedle patch, build a new photothermal therapy devices.
In recent years, by using MEMS technology, researchers developed a new medical equipment technology - microneedle. Polypyrrole (PPy) was mostly often focused on its conductive characteristic, but furthermore which has excellent photothermal conversion capability, light stability, and with its excellent biocompatibility, making PPy a very promising photothermal therapy material.
In our in vitro experiments, we adjusted the power of the near IR to manipulate the temperature. We used 0.5 W/cm2, 808 nm near IR with PPy microneedles to precede our experiment. In animal experiments, we demonstrate that this device has a good effect of bacterial inhibition. Prove the great potential of the PPy microneedle in photothermal therapy.

摘要 I
Abstract II
誌謝 III
目錄 IV
圖目錄 VI
第一章 緒論 1
第一節 光熱療法 1
第二節 光熱療法優勢 5
第三節 光熱療法載體 7
第四節 聚吡咯(Polypyrrole) 10
第五節 微生物感染與防治 12
第六節 微針貼片 14
第七節 研究目的 18
第二章 研究方法 21
第一節 光學模擬SU-8微針結構製程 21
第二節 SU-8微針結構製程 24
第三節 奈米聚吡咯粒子製備 26
第四節 利用灌模方式製作聚吡咯微針陣列 27
第五節 聚吡咯微針陣列壓應力穿刺測試 28
第六節 聚吡咯微針陣列光熱效應體外實驗 29
第七節 聚吡咯微針陣列光熱效應動物實驗 30
第八節 金黃色葡萄球菌抑菌實驗 32
第九節 光熱療法癒合實驗 34
第三章 研究結果 35
第一節 SU-8 微針結構 35
第二節 聚吡咯微針陣列壓應力穿刺測試 38
第三節 聚吡咯微針陣列光熱效應體外實驗 40
第四節 聚吡咯微針陣列光熱效應動物實驗 43
第五節 金黃色葡萄球菌抑菌實驗 45
第六節 光熱療法癒合實驗 47
第四章 結論 48
參考書目 50

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