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作者(中文):吳懷愛
論文名稱(中文):三維果蠅實驗平台之溫度控制與導入系統之自動化
論文名稱(外文):Temperature Control and Automatic Guiding System for 3D Experimental of Drosophila Platform
指導教授(中文):陳榮順
口試委員(中文):杜佳穎
蔡宏營
學位類別:碩士
校院名稱:國立清華大學
系所名稱:動力機械工程學系
學號:101033553
出版年(民國):103
畢業學年度:102
語文別:中文
論文頁數:57
中文關鍵詞:果蠅自動化溫度場
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本研究為三維果蠅實驗平台之溫度控制與導入系統之自動化,其內容分兩部分:溫度控制系統及自動化導入平台設計-含機構設計與自動化系統控制。本研究之第一部分主要探討果蠅之熱刺激環境的溫度場,採用中空玻璃球作為實驗環境,命名為生物球,熱源以數個薄型加熱片貼附於玻璃表面,並根據模擬結果假設實驗控制之加熱環境。環境監控平台設有八個溫度讀取點與四個加熱片,加熱片之溫度控制結果於40℃下之安定時間約4分鐘並可維持半小時之穩定狀態,最終穩態誤差小於1%。第二部分主要建立一自動化導入平台,透過自動化機構簡化果蠅實驗前之準備工作,以取代一部分人力,導入平台原型由壓克力與ABS塑膠製成,控制物件包括DC馬達、伺服馬達等,平台測試重點分有前段(刺激與計數)、後段(平移與導入),測試結果,計數正確率為81%、後段導入通過率為95%,整體實驗流程約5~10分鐘,可提供15隻果蠅/次導入至生物球。
摘要 I
誌謝 II
目錄 III
圖目錄 V
表目錄 X
第一章 緒論 1
1.1 前言 1
1.2 研究背景與動機 1
1.3 文獻回顧 3
1.4 論文架構 12
第二章 溫度模擬與量測系統 13
2.1 溫度場模擬 13
2.2 模擬結果 14
2.2.1 模擬不同尺寸之玻璃球 14
2.2.2 加熱模擬 15
2.3 溫度量測系統 23
2.3.1 量測溫度元件 23
2.3.2 溫度量測電路 24
2.3.3 資料擷取卡 25
2.3.4 LabVIEW人機介面 25
第三章 溫度控制系統與實驗架設 27
3.1 加熱片介紹 27
3.2 加熱控制原理 28
3.3 加熱電路 29
3.4 控制器設計 29
3.5 實驗系統架構圖 31
3.6 溫度感測器與加熱片黏貼位置探討 31
3.7 溫度控制實驗結果 33
第四章 果蠅導入系統之設計、實驗與結果討論 38
4.1 系統設計 38
4.2 系統零件 40
4.3 系統組裝 45
4.4 系統測試結果 50
第五章 結論與未來工作 54
5.1 結論 54
5.2 未來工作 54
參考文獻 55
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