本研究目的在測試碳纖維在神經移植的領域中是否具有發展性，實驗以PC12細胞作為模型，比較其在組織培養皿和碳纖維上之生長及分化情形，結果顯示，雖然碳纖維不具細胞毒性，PC12細胞也能順利貼附並成長於其表面，但碳纖維本身不具促進PC12細胞生長及分化的效果。
利用碳纖維導電性良好的特性，本研究設計一組通電裝置，對培養於碳纖維上之PC12細胞通以電刺激，探討透過碳纖維給予細胞電刺激所造成之影響。結果顯示，通以電壓為200 mV以上的電刺激會傷害細胞，電壓為100 mV的電刺激對細胞生存率不造成影響，但會使細胞神經突起沿著纖維方向生長。
對培養於碳纖維上，且以NGF誘導分化之PC12細胞通以不同時數的電刺激，結果發現電刺激無法促進神經突起的形成，但在神經突起的長度上，無電刺激組別的神經突起長度約為56 ± 4.8 μm，通連續24小時電刺激的組別，其神經突起的長度約為108 ± 6.8 μm，約為1.92倍，通連續48小時電刺激的組別，其神經突起的長度約為132 ± 5.1 μm，大約為2.35倍，此結果顯示電刺激的總時數越長，神經突起的長度則越長，證實在NGF的作用下，通電刺激對神經突起成長具有加成的效果。

The purpose of this study is to investigate the application of carbon fiber in neural implant. We used PC12 cells as a model, and compared the differences of proliferation and differentiation of the cells cultured in tissue cultured plates and on carbon fiber. The results revealed that although carbon fiber is a non-cytotoxic material which can be adopted as a substrate for normally proliferating PC12 cells to, however it cannot enhance the adhesion and proliferation of PC12 cells cultured on it.
Using the intrinsic property of high electrical conductivity of carbon fiber, we designed a device to provide the electrical stimulation to the PC12 cells cultured on carbon fiber and found that the electrical stimulation with potential over 200 mV is harmful to PC12 cells. The electrical stimulation with potential 100 mV makes no negative influence to the viability of PC12 cells, but can guide the neurites to outgrow along the carbon fibers.
Applying electrical stimulation to the NGF-induced differentiated PC12 cells cultured on carbon fiber, we found that electrical stimulation has very limited influence on the neurite formation, but it can enhance the elongation of neurites. Cells without electrical stimulation, the neurite length is 56 ± 4.8 μm μm, cells with 24 hours electrical stimulation, the neurite length is 108 ± 6.8 μm μm, about 1.92 times of the cells without electrical stimulation, and cells with 48 hours electrical stimulation, the neurite length is 132 ± 5.1 μm μm which is about 2.35 times of the cells without electrical stimulation. This results reveled that electrical stimulation has positive effect on neurtie growth with the addition of NGF.

第1章 緒論 1
1-1 神經系統 1
1-2 神經系統的退化 2
1-3 神經系統的修復 3
1-4 電刺激對神經細胞的影響 5
1-5 碳纖維 7
1-6 研究計畫 11
第2章 文獻回顧 21
2-1 細胞毒性 21
2-2 材料表面特性誘導細胞生長 23
2-3 電刺激促進細胞分化 25
2-4 人工神經束 29
2-5 碳纖維於生醫領域的應用 30
第3章 實驗方法 43
3-1大鼠腎上腺髓質嗜鉻細胞瘤細胞培養 43
3-1-1 細胞解凍 43
3-1-2 細胞繼代培養 46
3-2 通電裝置製作 49
3-2-1 碳纖維表面處理及分析 49
3-2-2 通電裝置製作 50
3-3 碳纖維對PC12細胞之影響 53
3-3-1 PC12細胞於碳纖維上之培養 53
3-3-2 碳纖維的細胞毒性分析 55
3-4電刺激對PC12細胞之影響 57
3-4-1 不同電壓值對PC12細胞生存率及分化率造成之影響 57
3-4-2 細胞分化 58
3-4-3 神經突起在碳纖維上之觀察 60
3-4-4 電刺激多寡對PC12細胞神經突起之影響 62
3-4-5 電刺激間歇性及總時數對PC12細胞神經突起之影響 63
3-4-6 電刺激加速PC12細胞神經突起之探討 64
3-4-7 長期電刺激對PC12細胞神經突起之影響 64
第四章 結果與討論 68
4-1 碳纖維表面分析 68
4-1-1 場發射掃描式電子顯微鏡觀察碳纖維表面形貌 68
4-1-2 傅立葉轉換紅外線光譜儀之分析結果 69
4-2 碳纖維對PC12細胞之影響 70
4-2-1 碳纖維對PC12細胞生長及分化的影響 70
4-2-2 碳纖維之細胞毒性測試 71
4-3 電刺激對PC12細胞之影響 72
4-3-1 不同電壓值對PC12細胞生存率及分化率造成之影響 72
4-3-2 碳纖維上通電刺激對PC12細胞神經突起生長方向之影響 73
4-3-3 電刺激多寡對PC12細胞神經突起之影響 75
4-3-4 電刺激間歇性及總時數對PC12細胞神經突起之影響 77
4-3-5 電刺激加速PC12細胞神經突起之探討 79
4-3-6 長期電刺激對PC12細胞神經突起之影響 79
第5章 結論 98
參考文獻 100

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