癌症為台灣十大死因之首，之所以難以治愈是因為癌細胞的轉移癌症所導致，在轉移的過程中產生「循環腫瘤細胞」（Circulating Tumor Cell, CTC）。近期研究顯示循環腫瘤細胞的多寡與病情的嚴重性和預測有正相關，因此偵測和分析循環腫瘤細胞是非常重要的研究，特別是對早期癌症轉移和接受藥物治療長期追蹤的病人。
本論文利用流體的特性，在載玻片上以SU-8做出結構，使懸浮細胞液內的細胞受液體流動的拉力而產生單層緊密排列的自組裝排列。與台中榮民總醫院合作取得乳癌第四期病患的血液檢體，經抗體螢光標定後利用重力和側向拉力的影響，在觀測區域形成二維陣列緊密排列。以螢光顯微鏡觀測循環腫瘤細胞並計算其數量再和流式細胞儀的結果對比。不同於一般實驗室細胞株的結果，具臨床意義且大量實驗後可建立有效的臨床數據。
此研究方法製作低成本且檢測快速，十分鐘內即可排列完成。不需要其他昂貴的檢測儀器，易取得的螢光顯微鏡便可進行偵測。主要成果包括：1.可實際運用於臨床上的檢測。2.螢光標定出的循環腫瘤細胞數量和流式細胞儀的結果相當。此外此檢測方法不只是用於乳癌病患，其餘癌症的轉移病患也可以此晶片檢測。

第1章 緒論 1
1.1 研究背景 1
1.2 研究目標 6
第2章 文獻回顧 7
2.1 細胞篩選方法 7
2.1.1 CellSearch系統檢測循環腫瘤細胞 7
2.1.2 免疫標定(Immunolabeling) 10
2.1.3 免疫標定應用於臨床實驗 15
2.1.4 非免疫標定(Non-immunolabeling) 21
2.2 文獻比較與目標 24
2.2.1 實驗目標 26
第3章 實驗設計 27
3.1 螢光檢測系統特色 27
3.2 實驗材料準備 28
3.2.1 細胞培養 28
3.2.2 藥品介紹 29
3.2.3 藥品製備處理 31
3.3 第一代微流道井式排列平台 33
3.3.1 第一代平台設計及原理 33
3.3.2 第一代平台製成及組裝 36
3.3.3 第一代微流道井式細胞排列結果簡介 38
3.4 第二代多孔晶片設計 41
3.4.1 第二代多孔晶片原理 43
3.4.2 兩代晶片比較 45
3.4.3 第二代自組裝陣列晶片細胞排列結果 46
3.5 全血於微流道井式細胞排列平台檢測 50
3.5.1 結論 52
3.6 轉移乳癌病患檢體於井式細胞排列平台檢測 53
3.6.1 病患檢體於自組裝細胞排列平台檢測結果 53
3.6.2 病患檢體於流式細胞儀檢測出的結果 58
3.6.3 結論 64
第4章 結論 65
4.1 第二代自組裝晶片結論 65
4.2 臨床檢體檢測結論 66
第5章 未來工作 68

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