本論文主要描述利用植物維管束平台於生化檢測之開發。本研究所使用之多元植物維管束平台為自然界含量豐富之資源、價格低廉、容易取得之材料; 甚至有些植物維管束平台是與我們生活息息相關之用具與產品。植物維管束能利用自然毛細作用力輸送流體物質，因此不需額外的能量供給幫浦。此外，植物維管束材料，例如：竹子及木頭，具有與其他基體材料(例如：紙或線基材)相同之極佳物理吸附特性，爰在塗布檢測試劑時不需複雜的化學表面修飾。再加上本實驗的製程僅需常見之機械加工，因此整個製造成本是相當低的。
本實驗中我們示範如何利用牙籤及咖啡攪拌棒作為主要之植物維管束平台材料以開發生化檢測裝置，其裝置之格式參照試紙及測流裝置之模式。為了驗證平台之可行性，我們設計一些不複雜的實施例，包括：葡萄糖檢測、膽固醇檢測、亞硝酸鹽檢測，及其半定量分析以茲証明。此外，我們期盼本研究可以影響公共衛生系統，更能將此影響拓展至台灣的傳統產業。由於台灣擁有世界首屈一指之工藝技術及製造技術(例如：三義木雕)，所以本團隊期望於此基礎上建立一套雙邊獲利之商業模式，以幫助台灣沉寂已久的傳統工業注入一股新的活水。









關鍵詞：植物維管束、攪拌棒、竹子、木頭

This thesis primarily describes the use of vascular bundle as a platform for the development of biochemical detections (i.e., public health related issues). The variety of vascular bundle platforms used for this research are abundance of nature resources, inexpensive easily accessible; some of them are well familiar daily instruments and products in ours living surroundings. Vascular bundle enables to transport fluids via capillary action, thus the additional energy for bumping is not required. In addition, vascular bundle materials such as bamboo and wood have the same extraordinary physical absorption prosperity as other matrix materials (e.g., paper or thread); no complicate chemical surface modification is necessary for immobilizing detection reagents. Also the fabrication of vascular bundle platforms just needs conventional mechanical processing. Therefore, the cost of the entire production is extremely low.
Here we demonstrate how to take advantage of toothpicks and coffee stirrers (both are easily accessible products) as our primary materials to forge versatile biochemical detection devices, whose format are similar with dipstick no less than lateral flow tests. In order to verify the feasibility of our designed ones, we attempt to perform some unsophisticated metabolic assays (e.g., glucose assay, cholesterol assay, and nitrite analysis), and also semi-quantitative analysis. Moreover, we expect our influence could extend to not only public health system but also Taiwan’s conventional industries. Because Taiwan has the state-of the-art technologies for process as well as manufacturing (e.g., Sanyi woodcarving), we hopes we could establish bilateral profit-making business models in the light of this foundation, which will be able to infuse new life into Taiwan’s traditional industries.

Keywords: Vascular bundle, coffee stirrers, toothpicks, bamboo, wood.

誌謝(Acknowledgements) I
摘要 II
Abstract III
目錄 (Table of Contents) IV
圖目錄 (List of Figures) VI
第一章 前言 (Introduction) 1
1.1 研究目的 1
1.2 植物維管束 1
1.2.1 木質部 4
1.2.2 韌皮部 5
1.3 檢測平台開發介紹 6
1.3.1 紙基材平台開發介紹 7
1.3.2 線基材平台開發介紹 9
1.4 亞硝酸鹽之危害 10
1.5 慢性疾病之重要性 11
1.5.1 葡萄糖檢測 12
1.5.2 膽固醇檢測 13
第二章 研究材料與方法 (Materials and Methods) 15
2.1 檢測試劑塗布於植物纖維管束之製程 15
2.1.1 檢測試劑塗布於牙籤之製程 15
2.1.2 檢測試劑塗布於竹製咖啡攪拌棒之製程 16
2.2 檢測試劑成分及濃度 17
2.3 實驗檢測原理 18
2.3.1 葡萄糖檢測原理 18
2.3.2 膽固醇檢測原理 18
2.3.3 亞硝酸鹽檢測原理 19
第三章 結果與討論 (Results and Discussion) 21
3.1 植物維管束毛細作用力探討 21
3.2 植物維管束微結構圖(掃描式電子顯微鏡) 21
3.3 植物維管束之牙籤平台驗證─葡萄糖檢測 24
3.4 植物維管束之牙籤平台驗證─膽固醇檢測 24
3.5 探討有機溶劑對植物維管束之影響 25
3.6 植物維管束內部流道可行性之驗證 26
3.7探討植物維管束之竹製及木製咖啡攪拌棒顯色效果 27
3.8探討植物維管束之凹槽設計與顯色效果關係 28
3.9探討植物維管束之V型凹槽設計位置與顯色效果關係 29
3.10探討植物維管束置於欲檢測樣本時間與顯色效果關係 31
3.11 植物維管束之竹製咖啡攪拌棒平台驗證─亞硝酸鹽檢測 32
3.12植物維管束之三維檢測平台之探討 34
第四章 結論 (Conclusions) 36
第五章 未來展望 (Future Work) 37
參考文獻 (Resferences) 38
附錄一 42

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