本研究在於關注大腸桿菌在不同環境條件下的生長和代謝特性，利用大腸菌兼性厭氧的特性，例如有氧環境下的呼吸作用 ( cellular respiration )，厭氧環境下的發酵作用 ( fermentation )，透過使用生物反應器進行培養及排放的二氧化碳濃度偵測，不同碳源之大腸桿菌培養實驗，觀察在有氧環境下其生長情形；而在厭氧環境下，目的在於觀察其透過發酵作用能產生氫氣多寡，透過培養條件的控制，比較其產生氫氣的差異，整個實驗硬體包含Arduino的微處理器來自動化偵測實驗數據，光密度測量 OD600、攪拌、氣體量測以及資料無線傳輸等。

The objective of this research is to examine the behavior and metabolism of Escherichia coli in various environmental conditions. By leveraging the fact that Escherichia coli can function both aerobically and anaerobically, the study employs a bioreactor to cultivate the bacteria and measure carbon dioxide concentrations during discharge. This study enables the examination of the metabolic activities of Escherichia coli under various conditions, such as its ability to carry out cellular respiration when oxygen is present and fermentation when oxygen is absent. Cultivation experiments using different carbon sources were conducted to observe Escherichia coli 's growth under aerobic conditions. Under anaerobic conditions, the aim is to observe the amount of hydrogen produced through fermentation and to compare the differences in hydrogen production through controlled cultivation conditions. The experimental hardware includes an Arduino microprocessor for automated detection of experimental data, such as optical density measurement at 600 nm, stirring, gas measurement, and wireless data transmission.

致謝 II
摘要 IV
Abstract V
目錄 VI
圖目錄 IX
表目錄 XI
一、 緒論 1
1-1研究動機 1
1-1-1全球氣候變遷 1
1-1-2研究目標 5
1-2文獻回顧 7
1-2-1呼吸作用 7
1-2-2發酵作用 9
1-2-3建構生物反應器 10
1-2-4二氧化碳的測定 11
1-2-5氫氣的測定 12
二、 實驗材料及方法 13
2-1實驗樣品與培養基的配置 13
2-2洋菜盤的製作及塗盤 15
2-3菌保的製作 16
2-4碳源配置 17
2-5細菌預養 18
2-6硬體裝置 19
2-6-1硬體簡介 19
2-6-2生物反應器的操作方式 24
2-7氣體條件及實現方式 26
三、 實驗結果 28
3-1實驗前準備 28
3-2實驗步驟 29
3-2-1二氧化碳與大腸桿菌生長實驗 29
3-2-2大腸桿菌產氫氣與二氧化碳實驗 30
3-3實驗結果 31
3-3-1大腸桿菌在有氧且不同碳源培養下的生長情形 31
3-3-2大腸桿菌在厭氧且不同培養基下產H2與CO2 34
四、 結論及討論 36
五、 參考文獻 37
六、 附錄 41
A-1. Arduino UNO程式碼 (二氧化碳與大腸桿菌生長實驗) 41
A-2. Arduino UNO程式碼 (大腸桿菌產氫氣與二氧化碳實驗) 50
A-3. 中英文對照表 63

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