由於去氧核糖核酸(DNA)與金屬離子獨特的鍵結特性，因此DNA被廣泛的作為奈米粒子還原的模板。在本研究中，我們以DNA為模板，利用光還原法製備金屬複合物，並討論其光學特性與刮刀成膜性質。在第一部分的研究中，我們製備出以DNA為模板的單金屬與多金屬奈米粒子溶液，並討論不同照光還原時間、混合順序及光還原劑作用下之吸收光譜與複合物之特性，優化後的結果亦同時分析與討論在本章節中。在第二部分的研究，我們利用刮擦的方式製備出以DNA為模板的單金屬與多金屬奈米粒子之薄膜，並利用暗視野顯微鏡及偏光顯微鏡來探討薄膜的表面影像與結構特性。結果顯示金屬奈米粒子可形成於刮擦法製備之薄膜上，研究中也以不同的製備條件來討論薄膜的表面結構特性之差異。本研究之結果展現運用DNA為模板的特性，能實現以簡易製程製備金屬奈米粒子複合物與薄膜，可進一步優化使用於光電元件與應用。

Deoxyribonucleic acid (DNA) has been widely employed as a template for the fabrication of nanostructures by virtue of its unique interaction with metal ions. In this study, we present the preparation and characterization of photoinduced DNA-templated metal hybrids and explore the film properties deposited by blade casting. In the first part, we present the studies of preparing DNA-templated metal nanoparticles (Ag, Au, Ag-Au) in the solution form. The properties of the nanoparticles are characterized by the absorption spectra with respect to different exposure duration, mixing order, and reagent. The optimized preparation conditions are analyzed and discussed. In the second part of the study, we present the film properties of DNA-templated hybrids deposited by blade casting. The film properties are characterized by the dark field spectroscopy and polarized optical microscopy. The results show that DNA-templated Ag, Au, Ag-Au nanoparticles are formed on the blade-casted films and the properties are discussed based on different preparation conditions. Our study presents a facile method based on the DNA-templated properties for the preparation of metallic nanostructures, which may further be optimized for the fabrication of functional photonic devices and applications.

致謝 I
摘要 II
Abstract III
圖目錄 VI
表目錄 XII
第一章 緒論 1
1.1 電漿子介紹 1
1.2 DNA薄膜的液晶特性 3
1.3 DNA鍵結金屬粒子的薄膜結構 4
1.4 研究動機 7
第二章 實驗方法 8
2.1 材料及樣品製備 8
2.1.1 調變DNA濃度溶液製備 8
2.1.2 光還原金、銀、金銀合金奈米粒子製備 8
2.1.3 DNA與合金鍵結薄膜製備 11
2.2 量測儀器與分析方法 12
2.2.1 紫外-可見光光譜儀 12
2.2.2 製備薄膜刮膜機 12
2.2.3 POM偏光顯微鏡 13
2.2.4 暗視野顯微鏡 14
2.3 影像處理與分析 15
2.3.1 合金薄膜的POM影像 15
2.3.2 表面週期性分析方法 15
2.3.3 合金表面的暗場影像 15
第三章 以DNA為模板之光還原金屬粒子製備與光學分析 17
3.1 以DNA為模板製備銀奈米粒子 17
3.1.1 比較照光時間還原AgNP的光學性質 17
3.1.2 鍵結光還原劑還原AgNP的光學性質 19
3.1.3 鍵結順序影響還原AgNP的光學性質 23
3.1.4 依照光時間還原AgNP效果分析 28
3.1.5 依混合順序還原AgNP效果分析 29
3.2 以DNA為模板組成金奈米粒子 32
3.2.1 不同光還原劑還原AuNP的光學性質 32
3.2.2 加入DNA還原AuNP的光學性質 33
3.2.3 DNA影響AuNP的還原探討 36
3.3 以DNA為模板組成金銀合金奈米粒子 37
3.3.1 還原不同合金濃度的光學性質 37
3.3.2 加入DNA還原不同合金濃度的光學性質 38
3.3.3 DNA影響不同合金濃度的還原效果探討 39
3.4 第三章小結 40
第四章 以DNA週期性結構鍵結金屬之薄膜探討 41
4.1 刮刀塗佈法製備DNA-Ag薄膜 41
4.1.1 DNA-Ag之薄膜的暗場量測 41
4.1.2 比較摻雜還原劑所製備之DNA-Ag膜POM分析 42
4.1.3 還原劑對DNA-Ag膜之週期性影響 47
4.2 刮刀塗佈法製備DNA-Au薄膜 49
4.2.1 DNA-Au之薄膜的暗場量測 49
4.2.2 不同還原劑對DNA-Au膜的POM分析 50
4.2.3 不同還原劑對DNA-Au膜的週期性討論 53
4.3 刮刀塗佈法製備DNA-AuAg薄膜 55
4.3.1 DNA-AuAg的暗場量測 55
4.3.2 比較溶液態鍵結DNA之DNA-AuAg膜POM分析 55
4.3.3 相較溶液態鍵結DNA對合金薄膜週期性影響 59
4.4 第四章小結 60
第五章 結果與未來展望本論文 62
參考文獻 64

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