本研究探討補充漢方中藥B307對ICR小鼠運動衰竭後引起肌肉損傷的緩解效果。以16隻ICR雄性小鼠依體重平均分成運動處理組（Control）、前處理組（Pre-treatment, Pre-T）、後處理組（Post-treatment, Post-T）及全處理組（Full-treatment, Full-T），每組各4隻。其中Pre-T組與Full-T組在游泳衰竭測驗前36小時開始給予餵食B307，劑量為50 mg/kg，餵食間隔為12小時，重複3次；另Control組與Post-T組則正常飲食與水。Pre-T組和Full-T組在第3次餵食後隔6小時，與Control組及Post-T兩組同時介入游泳衰竭運動測驗並記錄衰竭時間。Post-T和Full-T組則在測驗後1小時開始餵食處理，餵食間隔為12小時，重複3次。Post-T和Full-T組在最後餵食處理後隔12小時，與Control組與Pre-T組同時記錄體重，犧牲並採集血液、尿液及腓腸肌，以進行生化分析。研究結果顯示： Pre-T組與Full-T組的運動衰竭時間均顯著高於Control組與Post-T組。在衰竭後36小時，Control組的血液、尿液及骨骼肌均有顯著的發炎及損傷，而Pre-T、Post-T、及Full-T三組則均獲得顯著的緩解效果並有效提升抗氧化能力。即表示運動前給予餵食處理，能有效延長時間及提升肌耐力；不論運動前或後給予餵食處理，對運動衰竭引起的損傷，均有顯著改善與緩解效果。

The aim of this study was to investigate the effect of supplementing Chinese herbal medicine B307 on anti-oxidant, anti-inflammatory and muscle-relieving effects in ICR mice after exhaustive exercise. 16 ICR male mice were used as research subjects that were reared in the animal house for 1 week and given swimming practice. Body weight of mice were recorded at 30 hours before exhaustive exercise. Then mice were equally divided into 4 groups of Control, Pre-treatment (Pre-T), Post-treatment (Post-T), and Full-treatment (Full-T). The Pre-T and Full-T mice were given the herbal medicine B307 for feeding from 36 hours before the exhaustive exercise test. The feeding dose was 50 mg/kg, and the feeding period was 3 times that interval was 12 hours. Another Control and Post-T mice were given only normal diet and drinking water. Pre-T and Full-T mice were given a swimming exhaustive exercise at six hours after the final feeding of the herbal medicine B307, both Control and Post-T mice were given a swimming exhaustive exercise at the same time. The duration time of swimming exhaustive exercise each mouse was recorded during the test. The Post-T and Full-T mice were given the herbal medicine B307 treatment at 1 hour after the exhaustive exercise test, during which the feeding was performed 3 times that interval was 12 hours. Body weight of Post-T and Full-T mice were recorded at hours after the final feeding of the herbal medicine B307. Control and Pre-T mice were recorded at the same time. Then all mice were sacrificed to collect their blood, urine, and gastrocnemius muscles. Subsequent immunohistochemistry and biochemical analysis were performed. The results of this study showed that there was no significant difference in body weight regardless of Chinese medicine feeding treatment and swimming exhaustive exercise test or not. The duration time of exhaustive exercise in Pre-T and Full-T mice those fed with the herbal medicine B307 before exercise was significantly longer than those in Control and Post-T mice. Immunohistochemistry and biochemical analysis showed that Control mice had significant inflammation, oxidative stress, and damage in the blood, urine, and skeletal muscles that were examined at 36 hours after exhaustive exercise test, while significant relief effects were obtained in Pre-T, Post-T, and Full-T mice. The results suggested that the herbal medicine B307 treatment before exhaustive exercise can effectively prolong the muscular endurance in mice, while the herbal medicine B307 treatment both before and after exhaustive exercise has anti-oxidant, anti-inflammatory, and muscle-relieving effects in mice.

口試委員與系主任簽字之論文通過簽名表 ........................................ I
論文授權書 .................................................................... II
中文摘要 ............................................................................ IV
英文摘要 ............................................................................ V
謝 誌 .............................................................................. VII
目 次 ........................................................................... VIII
表 次 ............................................................................... XI
圖 次 .............................................................................. XII
第壹章 緒論 ....................................................................... 1
第一節 問題背景...................................................................1
第二節 研究目的.................................................................. 4
第三節 研究假設................................................................... 4
第四節 名詞操作型定義 ......................................................... 5
第五節 研究範圍與限制 ......................................................... 6
第貳章 文獻探討 .................................................................. 7
第一節 漢方中藥 B307 ........................................................... 7
第二節 衰竭運動..................................................................... 9
第三節 衰竭運動引發氧化壓力與發炎 ........................................ 10
第四節 衰竭運動引發組織的損傷 ............................................... 15
第五節 漢方中藥使用於緩解運動引發傷害的相關應用 .................... 16
第六節 文獻小結................................................................... 17
第參章 研究方法 .................................................................. 19
第一節 研究對象.................................................................... 19
第二節 研究材料與器材 ........................................................... 19
第三節 實驗時間與地點 ........................................................... 20
第四節 實驗設計..................................................................... 21
第五節 實驗流程..................................................................... 23
第六節 西方墨點法（Western blot, Wb） ................................... 24
第七節 免疫組織化學染色（Immunohistochemistry, IHC） ............ 26
第八節 劉氏染色（Liu Stain） ................................................. 28
第九節 尿液常規檢驗（Urine Routine） ..................................... 29
第十節 資料處理.................................................................... 29
第肆章 結果 ........................................................................ 30
第一節 不同時間餵食對體重變化的影響 ........................................ 30
第二節 不同時間餵食對衰竭時間變化的影響 .................................. 30
第三節 不同時間餵食對衰竭運動後抗氧化能力的影響 .................... 31
第四節 不同時間餵食對衰竭運動引發血液發炎反應的影響 ............ 32
第五節 不同時間餵食對衰竭運動引發尿液發炎反應的影響 ............ 33
第六節 不同時間餵食對衰竭運動引發骨骼肌發炎反應的影響 ........ 34
第七節 不同時間餵食對衰竭運動引發骨骼肌損傷反應的影響 ........ 34
第伍章 討論與結論 ............................................................... 36
第一節 餵食有效提升肌耐力及延長衰竭時間 .................................. 36
第二節 餵食有效緩解衰竭運動引發氧化壓力反應 ............................ 37
第三節 餵食有效緩解衰竭運動引發發炎反應 .................................. 38
第四節 餵食有效緩解衰竭運動後骨骼肌損傷反應 ............................ 40
第五節 結論 ........................................................................ 40
參考文獻 ........................................................................... 42
附錄 ................................................................................. 51
動物實驗同意書 ................................................................... 52
表 次
表 1 游泳衰竭運動 36 小時尿液常規檢查結果 ............................... 52
圖 次
圖 1 不同組別小鼠進行游泳衰竭運動測驗前後體重的變化 ................... 56
圖 2 不同組別小鼠進行游泳衰竭運動測驗達到運動衰竭的時間 ............. 57
圖 3 游泳衰竭運動 36 小時骨骼肌 SOD2 免疫組織化學染色結果 ......... 58
圖 4 游泳衰竭運動 36 小時骨骼肌 SOD2 蛋白質表現量 ................... 60
圖 5 游泳衰竭運動 36 小時劉氏染色結果 ...................................... 59
圖 6 游泳衰竭運動 36 小時骨骼肌 TNFα 免疫組織化學染色結果 .......... 61
圖 7 游泳衰竭運動 36 小時骨骼肌 NFκB 免疫組織化學染色結果........... 63
圖 8 游泳衰竭運動 36 小時骨骼肌 Vinculin 免疫組織化學染色結果 ...... 65

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