隨著科技日新月異，人們對各種事物都想要有更深入的研究，然而有時候的限制是來自技術的難關，受激拉曼散射顯微術是近幾年來生醫領域發展重要的新顯微術。受激拉曼散射顯微術有掃描生物活體的優勢，利用光學與分子的非線性效應，不需要在生物體內植入有害或有時效性的分子，將兩道光的頻率差調整至與分子振動頻率共振來偵測分子的存在。在此篇論文中，我們探討拉曼散射的基本原理、受激拉曼散射的條件、將受激拉曼散射概念應用到分子檢測術的各個步驟。其中關鍵包含脈衝重疊、脈衝強度調變、偵測系統的細節。最後一部分討論實驗上測試橄欖油、甲醇與丙炔醇的實驗結果和比較。目前的架構的解析度為數個微米，濃度偵測極限為0.1%。

Stimulated Raman scattering (SRS) microscopy is a useful technique for detecting molecules in live cells. It is a label-free imaging technique that could function well without changing the biological working condition. Raman scattering is a nonlinear optical process that happens when the input light scatters with certain molecules and photons are excited to higher vibrational or rotational energy levels. In this work, we demonstrate the construction of molecular detection technique applying stimulated Raman scattering. The resolution of the system is several microns. The sensitivity of detection is around 0.1% volume percentage concentration.

Abstract (2)
Chapter 1 Introduction (8)
Chapter 2 Theory (10)
2.1 Raman Scattering (10)
2.2 Spontaneous versus Stimulated Raman Scattering (11)
2.3 Coherent Raman Scattering Microscopy (13)
2.4 Comparison with Other Microscopic Techniques (14)
Chapter 3 Experimental Setup (15)
3.1 Delay Stage (17)
3.2 Acousto-Optic Modulator (21)
3.3 Lock-in Amplifier (23)
3.4 Objective Lens (26)
3.5 Photodetector (29)
Chapter 4 Experimental Results (30)
4.1 Dependence on Driving Intensity (32)
4.2 Dependence on Driving Concentration (36)
Chapter 5 Summary and Future work (38)
5.1 Summary (38)
5.2 Future Work (39)
References (40)

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