在本文研究中，提出以不同濃度的凝膠來製作一個平台，首先對粒徑60 nm與97 nm混合的螢光微珠進行雙向凝膠電泳實驗，以較強的垂直電場將所有微珠往出口驅動，再以較弱的水平電場將所有微珠往側向驅動，利用不同粒徑微珠造成的電泳速度差原理，將粒徑差距僅有37 nm的混合微珠在側向進行分離。也將此方法應用於分群胞外體，並分析在不同出口渠道的胞外體粒徑、數量分布，以及跨膜蛋白CD63標記的檢測，結果顯現在不同胞外體分群帶有不同的CD63表現量。

In this study, developing a platform with different concentrations of agarose gel to run orthogonal-field gel electrophoresis. In which orthogonal-field gel electrophoresis is applied to separate 60 nm and 97 nm fluorescent beads depends on their different electrophoresis mobility. A stronger vertical electric field drives all beads toward the outlet wells, while a weaker horizontal electric field is applied alternatively and used to separate the mixed beads that differ by only 37 nm in diameter. This method is also applied to separate extracellular vesicles (EVs) into different subgroups. The size profile and concentration of EVs harvested in different outlet wells have been characterized. It is found that, CD63 molecules are expressed differently in EV subgroups.

摘要
Abstract
目錄
中英文名詞對照表 -------- 9
一、緒論 -------- 12
二、實驗材料與方法 -------- 25
三、實驗結果與討論 -------- 36
四、未來展望 -------- 60
五、參考文獻 -------- 61

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