金奈米棒吸收光能後得以熱緩解至環境，且其長軸表面電漿共振吸收波長可藉由增加長寬比調變至近紅外光區。由於此波長可以穿透至生物組織深處，故使金奈米棒可廣泛應用於光熱治療中。在過去的研究中，常以紅外線熱像儀擷取的生物體表面溫度變化，評估光熱治療的效果。然而在光熱治療的過程中組織內部的溫度通常高於生物體表面的溫度，故可能低估病灶的局部溫度而導致病灶周圍的健康組織受到熱傷害。因此，吾人將金奈米棒固定於瓊脂內1 mm3的空間中，以兩種近紅外光源，808 nm連續式雷射及850 nm發光二極體，激發樣品中的金奈米棒做為熱源，用以模擬生物組織進行光熱治療的過程，並搭配紅外線熱像儀，其時間、空間與溫度解析度分別為0.16 s-1、50 μm與0.04 °C，記錄樣品表面的溫度於時間與空間演進。
吾人藉由加熱過程中表面溫度變化量之空間分佈呈現對稱性，確認樣品無熱對流的現象。吾人亦將兩種近紅外光源之照射功率依瓊脂的散射程度修正為有效功率，並依吾人建立之點熱源熱傳導模型擬合以兩種近紅外光源照射樣品30秒時沿Z軸之溫度變化量空間分布，可獲得和實驗製備加熱源一致的深度。吾人於本研究提出以非接觸式的方法進行加熱與偵測，並對加熱物體表面溫度的空間分佈進行分析的方法，預期可應用於分析光熱治療時組織內部的熱傳導模型。

Gold nanorods (AuNR) offer a tunable longitudinal surface plasmon resonance band in near infrared region for the applications in photothermal therapy. Generally, the temperatures evolution of the object of interest upon photothermal treatment was recorded by the thermographic imaging of the object surface. However, when the object surface reached the curable temperature, the interior temperature will be higher than the required and cause further damage of the healthy cells or tissues at the vicinity of the unhealthy areas. In this work, the agar was chosen to serve as a bio-mimicking tissue and a point heat source model was constructed to evaluate the accurate inner temperature though monitoring the surface temperature. An agar matrix was embedded with an 1 mm3 AuNR agar cube in different depths with respect to the agar surface. The photoexcitation of AuNR agar cube with 808 nm CW laser or 850 nm LED light leads to the evolution of increasing surface temperature monitored by an infrared thermographic camera. The temporal resolution, spatial resolution and temperature resolution in our system are 0.16 s-1、50 μm and 0.04 °C, respectively.
The symmetric distribution of the temperature change along the x and z axes through the heating center suggested that the sample is non-fluidic and the convection and the mass flow can be excluded. The injection powers of laser and LED light were corrected according to the absorption and scattering of the agar matrix for fitting the temperature distribution along the z axis after heating for 30 seconds using the point source heat transfer model. The depths of the AuNR agar cube can be determined and are consistent with the prepared depths. Thus, I reported that the development of a bio-mimicking matrix using agar upon optical heating of the embedded AuNR sample and thermographic monitoring is feasible to the analysis of the surface and interior temperature evolution.

第一章 緒論 1
1.1 前言 1
1.2 光熱治療簡介 1
1.3 實驗動機 3
參考文獻 4
第二章 文獻回顧 7
2.1 金奈米棒之光學性質 7
2.1.1 侷域性表面電漿共振 7
2.1.2 光熱效應 8
2.2 非接觸式測溫方式 9
2.2.1 光聲測溫 10
2.2.2 螢光溫度計 11
2.2.3 紅外線熱像儀 11
2.3 熱傳導反問題 12
2.3.1 熱傳導係數 12
2.3.2 熱源位置與加熱功率 13
2.4 瓊脂熱傳導性質 14
參考文獻 25
第三章 儀器原理、實驗系統架設、實驗樣品製備及點熱源模型建立 30
3.1. 儀器原理介紹 30
3.1.1. 靜態紫外/可見/近紅外光吸收光譜 30
3.1.2. 穿透式電子顯微鏡 31
3.1.3. 紅外線熱像儀 32
3.2. 紅外線熱像儀系統架設 33
3.3. 樣品合成與製備 34
3.3.1. 金奈米棒合成、純化與濃縮 34
3.3.2. 瓊脂樣品製備 35
3.3.3. 實驗藥品 36
3.4. 點熱源模型 36
3.5. 儀器參數設定 39
3.5.1. 靜態紫外/可見/近紅外光吸收光譜 39
3.5.2. 穿透式電子顯微鏡 39
3.5.3. 紅外線熱像儀系統 40
參考文獻 50
第四章 實驗結果與討論 51
4.1. 金奈米棒之靜態光譜及形貌 51
4.1.1. 金奈米棒的靜態可見/近紅外光譜 51
4.1.2. 穿透式電子顯微影像 52
4.2. 點熱源模型之修正 52
4.3. 數據處理 52
4.3.1. 扣除背景 53
4.3.2. 扣除瓊脂受光加熱所造成的升溫 53
4.3.3. 溫度變化量空間分布圖之對稱性 53
4.3.4. 加熱功率修正 54
4.3.5. 以點熱源模型擬合溫度變化量空間分布並模擬溫度變化量空間分布 54
4.4. 瓊脂表面的溫度演進 55
4.4.1. 808 nm雷射激發樣品之XZ平面溫度變化量空間分布演進 55
4.4.2. 850 nm發光二極體激發樣品之XZ平面溫度變化量空間分布演進 56
4.5. 利用點熱源解熱傳導反問題 57
4.5.1. 實驗樣品不具流動性 57
4.5.2. 808 nm雷射照射樣品之溫度變化量空間分布擬合與模擬結果 57
4.5.3. 850 nm發光二極體照射樣品之溫度變化量空間分布擬合結果 58
參考文獻 86
第五章 結論 87

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