本篇研究主要是探討腦腫瘤細胞與腦內環境的巨噬細胞的交互作用，在本實驗室過去的研究有發現，在腦腫瘤生長的過程中常會伴隨大量進潤的巨噬細胞，這些巨噬細胞在腫瘤生長過程中扮演著中要的角色，而過度繁殖和具有入侵能力的腫瘤細胞常是造成之治療失敗的原因，在本篇研究也發現在體外系統中，當腦腫瘤細胞與巨噬細胞共培養後，會使得腦腫瘤細胞聚集的生長在一起，而這些球狀的腫瘤細胞也表現出與原來腫瘤細胞不同的特性，對於放射治療與藥物治療也有不同的反應。

Excessive proliferation and high invasive ability are prime accused for the failure on current brain tumor therapy. Our previous study has shown that the recurrent brain tumors are frequently associated with higher content of tumor-associated macrophages (TAMs). This study aimed to explore the interaction between macrophage and brain tumor cell and delineate their role on brain tumor response to therapy. Initially, we measured the migration rate of a murine astrocytoma cell line, ALTS1C1. We found that the migration rate of ALTS1C1 could be increased if they were co-cultured with macrophages without direct cell contact. On the other hand, we were surprised to find that tumor cells were restrained within a spheroid like structure by macrophages when these two cells were cultured together. The macrophage-promoted tumor cell colonization also affect their response to the cytotoxicity of radiation.

摘要………………………………………………………………………………………………………………………p.1
誌謝………………………………………………………………………………………………………………………p.2
目錄………………………………………………………………………………………………………………………p.3
Chapter1 Introduction……………………………………………………………………….p.4
1.1 Brain tumor……………………………………………………………………………………….p.4
1.2 Microglia…………………………………………………………………………………………….p.4
1.3 Tumor microenvironment……………………………………………………………p.5
1.4 Radiation therapy…………………………………………………………………………p.6
1.5 Chemo-drug therapy………………………………………………………………………p.7
1.6 3D cell culture……………………………………………………………………p.7
Chapter 2 Material and method……………………………………………………....p.9
2.1 Cell culture……………………………………………………………………....p.9
2.2 Immunofluorescence……………………………………………………....p.10
2.3 Bone marrow derived macrophagedistraction…p.13
2.4 MTT assay…………………………………..p.14
Chapter 3 Result…………………………………………………………………………………………………..p.15
Chapter 4 Discussion………………………………………………………………………………………….p.22
4.1 Tumor migration………………………………………………………………………….p.22
4.2 Proliferation…………………………………………………………………………………p.23
4.3 DNA damage……………………………………………………………………………………..p.23
4.4 3D cell culture………………………………………………………………………..p.24
Grapic…………………………………………………………………………………………………………………......p.26
References……………………………………………………………………………………………………………....p.54
 

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