利用銨離子在鹼性環境中轉換成不帶電荷的氨之特性，可以建立一套簡單的吹氣捕捉前處理系統，排除複雜的高鹽基質干擾，再利用離子層析儀量測銨離子。在前處理系統中，使用高濃度氫氧化鈉可以製造一個鹼性環境，並且減少銨離子和水之間的水合作用。然而在量測含有胺基酸樣品時，會因強鹼的環境造成胺基酸水解產生額外的氨，進而影響量測準確度。本論文主要目的為，透過使用吸水性物質，減少銨離子和水之間的水合作用，藉此達到降低氫氧化鈉使用濃度之目的。
在本系統中，測試之吸水性物質有氯化亞鈷、硫酸鎂、氧化鈣、聚丙烯酸、分子篩以及多種親水性有機溶劑等，其中甲醇之使用可使加入的氫氧化鈉濃度降低一個數量級，並維持相同吹除效率。實驗中，透過改變溶劑含量、吹除瓶內樣品和氫氧化鈉比例、樣品配製方法、吹除時間以及系統溫度等，探討對吹除效率之影響。經改良後的吹氣捕捉系統，在最佳實驗參數條件下，可得吹除效率為96%。在量測胺基酸樣品時，因強鹼的環境造成胺基酸水解產生的氨濃度，與先前系統相比，減少達1.6到10倍。

Ammonium ion can convert to ammonia under alkaline environment. Based on this characteristic, ammonium ion can be efficiently separated from the interference of high concentration of salt with purge and trap system and quantitatively measured by Ion Chromatograph. The use of high concentrations of sodium hydroxide in the pretreatment system can produce an alkaline environment and reduce the hydration between ammonium and water. However, in the measurement of amino acid-containing samples, the hydrolysis of amino acids produces additional ammonia and affects the accuracy of results. The main purpose of this paper is to reduce the hydration between ammonium and water by using a water-absorbing substance, so that the concentration of sodium hydroxide used in the pretreatment system can be reduced.
This study has tested several water-absorbing substances, such as cobalt chloride, magnesium sulfate, calcium oxide, polyacrylic acid, molecular sieves and a variety of hydrophilic organic solvents. With the same purging efficiency, the use of methanol can reduce the concentration of sodium hydroxide by an order of magnitude. The purging efficiency with the addition of methanol was investigated by changing the proportion of the sample and sodium hydroxide in the purging bottle, the sample preparation method, purging time, and temperature of the system. The improved pretreatment system has greatly improved the detection limits of ammonia concentration in amino acid-containing samples up to 1.6 to 10 times less than previous systems.

中文摘要 I
英文摘要 II
致謝 III
目錄 IV
圖目錄 VI
表目錄 IX
第一章 前言 1
1.1 簡介 1
1.2 研究動機 2
第二章 文獻回顧 3
2.1 氨的基本物理化學特性 3
2.2 液相氨的分析方法 5
2.3 亨利定律 12
2.4 吹氣捕捉系統模式探討 14
2.5 吸水性物質 21
第三章 實驗方法 23
3.1 儀器設備 23
3.2 實驗藥品 25
3.3 前處理系統 27
3.4 前處理系統吹除效率的實驗流程 29
第四章 結果與討論 30
4.1 吸水性物質測試 30
4.1.1 矽膠乾燥劑 ( silica gel ) 30
4.1.2 分子篩 (molecular sieves ) 30
4.1.3 聚丙烯酸 ( polyacrylic acid, PAA ) 33
4.1.4 無水硫酸鎂 (MgSO4) 33
4.1.5 氧化鈣 (CaO) 34
4.1.6 親水性有機溶劑 34
4.2 效率參數之探討 37
4.2.1 溶劑含量之探討 37
4.2.2 樣品配製方法之探討 38
4.2.3 吹除瓶內樣品和氫氧化鈉比例之探討 40
4.2.4 吹除時間之探討 42
4.2.5 溫度之探討 46
4.3 檢量線、偵測極限、再現性 48
4.4 氨基酸樣品測定 51
第五章 結論 56
第六章 未來展望 56
參考文獻 57
附錄一 不同有機溶劑在不同氫氧化鈉濃度下之檢量線圖 64
附錄二 不同變因下之檢量線圖 67
附錄三 OPA / Na2SO3螢光衍生試劑最佳化條件探討 71

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