本研究的目標包含(1)研究柵藻於兩種不同刺激狀態下提升微藻體內之油脂含量的效率，第一種為缺乏氮源以及第二種為加入暗發酵產氫菌廢液刺激。(2)利用柵藻處理暗發酵產氫菌的廢液的可能性。暗發酵產氫菌生可產出合成氣體，是未來的能源發展前景，但暗發酵產氫菌生產合成氣體的同時，留下許多可再利用含有機酸廢液，這些有機酸有潛力可作為柵藻異營培養時的碳源來源。。
本研究以控制組、缺氮組、0.3x廢液組以及0.5x廢液組進行實驗比較，並利用柵藻於缺氮源刺激與暗發酵產氫菌廢液刺激之生長、色素含量、油含量以及培養液中有機酸含量來決定處理程序的效率。控制組之生物量產量為0.155g / L/day，其油脂含量達30.2wt％。缺氮組生產之生物量產量為0.155g / L /day，而其油脂含量為32.6wt％。0.3x廢液組生物量產量為0.177g / L /day，其油脂含量高達43.73wt ％。0.5x廢液組生物量產量為0.085g / L /天，其油脂含量為15.13wt ％。
由實驗結果獲知利用0.3x廢液刺激柵藻，其生物量生產力生與油脂含量最高。除此之外，0.3x廢液對暗發酵產氫菌廢液有機物之移除率較0.5x廢液組高，其中乳酸、甲酸、乙酸、丙酸和丁酸的移除率為84.01 %、100 %、100 %、84.01 % 和67.69 %。

This study aims to study the effects of two stress, nitrogen deprivation and organic acid addition, on the metabolism products of Scendesmus abundans. The organic acids were produced by the dark fermentation of hydrogen production bacteria. Dark fermentation is a future prospect for the synthesis of renewable fuel gas (syngas) but its effluent contains many organic acids which are generally considered as waste products.
Four different sets of experiments have been conducted: control (sufficient nitrogen), nitrogen depletion, 0.3 x effluent addition, and 0.5x effluent addition. Biomass productivity, pigment yields, lipid contents, and organic acid removal rates were compared to select the optimal stress condition. Biomass productivity produced by the control set was 0.155 g/L/day and lipid abundance was 30.2 wt%. Nitrogen deprivation produced a biomass productivity of 0.155 g/L/day and a lipid abundance of 32.6 wt%. The addition of 0.3x effluent produced a biomass productivity of 0.177 g/L/day and a lipid abundance of 43.73 wt% while 0.5x effluent produced a biomass productivity of 0.085 g/L/day and a lipid abundance of 15.13 wt%. These results show that 0.3x effluent of the hydrogen production fermentation had the highest biomass productivity and lipid abundance compare to the others. Besides, 0.3x effluent also has a better potential to decrease amounts of organic acids comparing to 0.5x effluent. 0.3x effluent removal efficiency of lactic acid = 84.01 %, formic acid = 100 %, acetic acid =100 %, propionic acid = 84.01 % and butyric acid = 67.69 %。

摘要 2
Abstract 4
致謝 6
目錄 I
圖目錄 IV
表目錄 1
第1章 緒論 2
1.1 研究動機與目的 2
1.2 實驗架構 3
第2章 文獻回顧 4
2.1 微藻培養介紹 4
2.1.1 微藻生長週期 5
2.1.2 微藻光合作用 6
2.2 微藻生產生質柴油 7
2.3 影響微藻生物質生物質與油脂含量 9
2.3.1 溫度 9
2.3.2 pH值 10
2.3.3 鹽度 12
2.3.4 光 13
2.3.5 氮源、磷源和硫源 15
2.3.6 碳源 17
2.4 微藻的廢水處理 22
2.5 暗發酵產氫細菌 25
第3章 實驗方法與材料 37
3.1 本實驗的簡易流程圖 37
3.2 柵藻培養 37
3.2.1 儀器與耗材 37
3.2.2 母瓶培養 （固轉液） 39
3.2.3 柵藻洋菜膠培養（固轉固） 44
3.3 暗發酵產氫菌 45
3.3.1 儀器與耗材 46
3.3.2 暗發酵細菌培養基配置 48
3.3.3 連續式暗發酵產氫發酵系統 50
3.4 Two layer Photo bioreactor（PBR）培養系統 54
3.4.1 儀器、藥品與耗材 54
3.4.2 生長期（Growth phase） 56
3.4.3 油脂累積（Lipid accumulation） 57
3.4.4 色素之萃取 (Pigment extraction) 57
3.4.5 油脂之萃取 （Lipid extraction） 59
3.4.6 高效液相層析儀分析暗發酵產氫菌有機酸 60
第4章 初步結果與討論 62
4.1 暗發酵產氫菌細菌培養 62
4.1.1 羊毛氈對連續式產氫的影響 62
4.1.2 外加羊毛氈的連續式產氫在不同溫度的培養 63
4.1.3 暗發酵細菌理論產氫 64
4.2 柵藻生物質檢量線 67
4.3 柵藻生物質的生長期階段 67
4.4 柵藻於累積油脂階段之生物質與色素分析 70
4.5 柵藻對暗發酵產氫菌廢液的有機物處理 71
4.6 柵藻在不同培養基的油脂含量分 72
4.7 結論 74
第5章 未來展望 79
5.1 不同種微藻累積油脂 79
5.2 暗發酵產氫菌廢液濃度 79
5.3 缺營養累積油脂 79
5.4 多樣性刺激培養 79
參考文獻 81

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