此篇研究整合了一個專門檢測過敏微陣列晶片的自動化微流體系統。過敏已經是現下社會中常見疾病之一，其發生是因為人體的免疫系統會對於周遭環境的過敏原產生過敏反應。然而，現行的過敏檢測需要訓練有素的人員手動來操控相對繁複的流程。本論文結合了微流道技術和微陣列晶片來開發一個微型過敏檢測晶片的整合性微流體系統。從我們先前的實驗中，可以證實一個在微陣列晶片上檢測過敏的微流道系統是可行的。經過大量製造單區反應晶片並優化相關參數後，顯示其螢光訊號在反應時間減少了三分之一後仍可達到現行手動晶片的強度。為了有效的降低成本以及簡化流程，本研究開發了一個可同時多樣本的全新過敏檢測系統，實驗結果證實了多區反應可以獲得相似的訊號。未來利用此微流體系統在微陣列過敏晶片上自動化多重檢測不只可以達到現行手動晶片的訊號強度，還有機會可獲得更多的生物應用。

This study reports an integrated automatic microfluidic system for detection of allergy in a microarray platform. Nowadays, allergy is very common in the world. It is a response of the human immune system to antigens in the surroundings. However, detection for allergy requires tedious manual operation, well-trained technicians and a relatively labor-intensive process. In the study, an integrated microfluidic system has been developed by combining several microfluidic techniques and microarray chips. Our previous experiments showed that a microfluidic system for allergy-diagnosis on microarray chips could be realized. It was demonstrated that after mass fabrication, the use of optimized conditions could obtain the similar fluorescence signals to the ones from manual processes and it only took only 20 minutes for the single reaction zone. In order to cut down costs and simplify the process, a new microfluidic system capable of simultaneous multiplex allergy-diagnosis was developed in this work. Experimental results demonstrated that multiplex reaction zones showed similar signals. Not only could it obtain the signals comparable to the on-bench experiments, but it also could increase the applicability of the integrated microfluidic system for automating multiplex detection on allergy microarray chips.

Abstract I
摘要 II
致謝 III
Table of contents V
List of Tables VII
List of Figures VIII
Abbreviations XII
Chapter 1 Introduction 1
1.1 MEMS and microfluidic technology 1
1.2 Background and literature survey 2
1.2.1 Allergy 2
1.2.2 ELISA and microarray chip 4
1.2.3 Microfluidic devices 4
1.3 Motivation and novelty 6
1.4 The Structure of thesis 8
Chapter 2 Materials and methods 9
2.1 Chip design and fabrication 9
2.2 Preparation of the experimental materials 16
2.3 Experimental procedure 16
2.4 Experimental setup 18
Chapter 3 Results and discussion 22
3.1 Characterization of the microfluidic chip with single reaction zone 22
3.1.1 Chip structure 22
3.1.2 Reaction optimization and signal analysis 24
3.2 Characterization of microfluidic chip with multiple reaction zones 28
3.2.1 Chip structure 28
3.2.2 Pumping rate of micro-pump 33
3.2.3 Reaction optimization and signal analysis 36
3.3 Comparison between on-bench and on-chip processes 42
3.3.1 Single reaction zone on microarray chip 42
3.3.2 Multiple reaction zones on microarray chip 44
Chapter 4 Conclusions and future works 46
4.1 Conclusions 46
4.2 Future perspective 47
4.2.1 Optimize the signal and CV 47
4.2.1 Clinical samples detection 47
References 48

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